摘要
松针黄酮类化合物药理作用显著,在抗氧化、神经系统保护等方面都有所表现,但其易受到外界因素影响,稳定性差。本研究对松针总黄酮微胶囊的制备优化条件及对其辅助改善帕金森(Parkinson's disease即PD)的作用进行了研究。采用微胶囊技术,用β-环糊精包裹松针总黄酮,通过正交实验优化条件,再通过检测不同浓度的微胶囊样品对分化后的SH-SY5Y细胞的损伤干预作用和羟自由基清除能力来初步研究其对辅助改善PD的作用效果。结果显示:当芯壁比为15,搅拌温度为40℃,搅拌时间为60 min时所制得微胶囊较好,包埋率可达51.37%。采用6-OHDA损伤SH-SY5Y细胞建立帕金森体外细胞模型,松针总黄酮微胶囊在放置60 d后,给药组细胞活力明显升高,且其对羟自由基有较好的清除作用,表现优于VC。因此,本研究制备的松针总黄酮微胶囊可作为辅助改善PD的功能性产品进一步研究开发。
Flavonoids of pine needles feature significant pharmacological actions,and certain effect in anti-oxidation,protection of the nervous system and so on.However,they are vulnerable to external factors and are of poor stability.This paper is a preliminary study on the optimization of preparation conditions of total flavonoids of pine needles microcapsules and the assistant effect of the same on Parkinson's Disease(PD).Such conditions were optimized with micro-encapsulation technology and through orthogonal experiment withβ-cyclodextrin wrapping up total flavonoids of pine needles.After that,by measuring the intervention effect of microcapsule samples of different concentrations on differentiated SH-SY5Y cells and their hydroxyl radical scavenging ability,their effect on ameliorating PD was analyzed preliminarily.The results indicated that:at the core-wall ratio of 15,the stirring temperature of 40℃and the stirring time of 60min,prepared microcapsules were better,with a micro-encapsulation efficiency up to 51.37%.By building an in vitro model of PD with 6-OHDA damaged SH-SY5Y cells and placing total flavonoids of pine needles microcapsules for 60 days,it was noticed that,cells with drug administration showed significantly higher viability,and better hydroxyl radical scavenging performance than VC.Therefore,the total flavonoids of pine needles microcapsules prepared in this research can be further developed as a functional product with assistant therapeutic effect on PD.
作者
曹侃
CAO Kan(School of Bioengineering,Wuhu Institute of Technology , Wuhu 241000,China)
出处
《武汉轻工大学学报》
2020年第6期17-22,共6页
Journal of Wuhan Polytechnic University
基金
安徽省教育厅自然科学重点项目(KJ2018A0694)
安徽省质量工程教学创新团队项目(2019cxtd023)
安徽省省级大规模在线开放课程(MOOC)示范项目(2018mooc494)
安徽省省级高水平高职专业项目(2018ylzy156).
关键词
松针黄酮
微胶囊
PD
pine needle flavonoids
microcapsules
PD
