摘要
目的建立驱虫斑鸠菊中异绿原酸A、异绿原酸C的含量测定方法。方法 Shim-pack VP-ODS C_(18)柱(4.6 mm×250 mm,5μm),甲醇-乙腈0.4%磷酸溶液(13.5∶13.5∶73)为流动相,流速:1 mL·min^(-1),柱温35℃,检测波长323 nm,进样量:10μL。结果异绿原酸A浓度在5.825～69.9μg·mL^(-1)与峰面积呈良好的线性关系,异绿原酸C浓度在5.15～61.80μg·mL^(-1)与峰面积呈良好的线性关系,二者加样回收率分别为98.70%～101.92%(RSD=1.04%,n=9)、95.99%～102.52%(RSD=1.90%,n=9)。结论该方法简便、快速、准确,可用于驱虫斑鸠菊中异绿原酸A、C含量测定及药材质量控制。
Objective To establish the chromatographic conditions of isochlorogenic acid A and isochlorogenic acid C in vernonia anthelmintica.Methods By changing the mobile phase,flow rate,column temperature and other chromatographic conditions,the best chromatographic conditions was we pursued to established.Results The linear relationship between the concentration of isochlorogenic acid A and the peak area was between 5.825-69.9 μg·mL -1 ,and the concentration of isochlorogenic acid C,was between 5.15-61.80 μg·mL -1 and the peak area was good .The sample recovery rates of the two groups were 98.70%-101.92%（RSD=1.04%, n =9）、95.99%-102.52%（RSD=1.90%, n =9）.Conclusion The method is simple,rapid,accurate and reliable for the determination of isochlorogenic acid A and isochlorogenic acid C in Vernonia anthelmintica and also for the quality control of the raw material.
作者
陈嘉媛
霍仕霞
闫明
宋志媛
斯拉甫.艾白
CHEN Jiayuan;HUO Shixia;YAN Ming;SONG Zhiyuan;SILAFU·Aibai(College of Pharmacy,Shihezi University,Shihezi 832003,China;Xinjiang Institute of Traditional Uighur Medicine,Xinjiang Laboratory of Uighur Medical Prescription,Urmuqi 830049,China)
出处
《医药导报》
CAS
北大核心
2018年第11期1393-1396,共4页
Herald of Medicine
基金
新疆维吾尔自治区重点研发计划项目(2016B03038-3-3)
关键词
驱虫斑鸠菊
异绿原酸A
异绿原酸C
色谱法
高效液相
Vernonia anthelmintica；Isochlorogenic acid A；Isochlorogenic acid C；Chromatograph
high performance liquid
