摘要
目的:建立以高效液相色谱法测定驱虫斑鸠菊中紫铆素含量的方法。方法:色谱柱为HypersilODS(250mm×4.6mm,5μm),流动相为甲醇-0.5%磷酸溶液(40∶60),检测波长为280nm。结果:紫铆素进样量在0.01～0.5mg范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为93.6%,RSD=2.89%(n=9)。结论:本法简单、准确,可用于驱虫斑鸠菊的质量控制。
OBJECTIVE： To establish an HPLC method for determination of butin in Vernonia anthemintica . METHODS： The samples were determined on Hypersil ODS column（250mm×4.6mm,5μm）, the mobile phase consisted of methanol- 0.5% HaPO4 （40 ： 60） and the detection wavelength was set at 280 nm. RESULTS： The linear range of butin was 0.01-- 0.5 rag（ r = 0. 999 9） and the average recovery was 93.6% （RSD = 2.89%, n = 9） .CONCLUSION ： The method is simple and accurate,and it is applicable for the quality control of butin in V.anthelmintica.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第30期2366-2367,共2页
China Pharmacy
