摘要
建立的高效液相色谱法同时测定水果中柠檬黄、新红、苋菜红、胭脂红、日落黄、诱惑红、酸性红7种人工合成色素。样品经提取后,采用C18反相色谱柱分离,以20mmol/L乙酸铵-甲醇为流动相进行梯度洗脱,柱温35℃,流速0.8m L/min,进样量10μL,采用二极管阵列检测器进行检测。以目标物的色谱保留时间和光谱图进行定性,以色谱峰的峰面积用标准曲线外标法进行定量。在优化的色谱条件下,7种色素的线性范围为0~100μg/m L,相关系数均大于0.999。各目标物的加标回收率为95.8%~103.9%,相对标准偏差均小于4%(n=6)。方法准确、快速、灵敏,适用于水果中违规添加合成色素的检测。
A high performance liquid chromatographic method was developed for the simultaneous determination of
seven synthetic pigments （tartrazine, new red, amaranth, ponceau, sunset yellow, seduction red, and acid red） in
fruits. After extraction, the fruits samples were separated on a C18 （3.5μm, 4.6mm×150mm） reversed-phase
chromatographic column with 20mmol/L ammonium acetate-methanol as the mobile phase for gradient elution. The
column temperature was 35℃, the flow rate was 0.8mL/min, and the sample volume was 10μL. Diode array detector
was used for detection. The chromatographic retention time and spectrogram of the target compound were used to
determine the quality of the product, and the peak area of the chromatographic peak was determined by standard curve external standard method. Under the optimized chromatographic conditions, the linear ranges of the seven
pigments were 0~100μg/mL, and the correlation coefficients were all greater than 0.999. The recoveries of all targets were 95.8%~103.9%, and the relative standard deviations were less than 4%（n=6）. The method is accurate, rapid and sensitive, and is suitable for the determination of synthetic pigment in fruit illegally added.
作者
刘佩
于文江
郑红
LIU Pei;YU Wen-jiang;ZHENG Hong(Shandong Institute for Food and Drug Control,Jinan 250103,China)
出处
《中国果菜》
2018年第8期11-13,共3页
China Fruit & Vegetable
关键词
高效液相色谱
水果
合成色素
High performance liquid chromatography
fruits
synthetic pigments
