摘要
目的探讨调脂治疗对血脂异常大鼠氧化应激、炎症因子及肾的影响。方法将42只Wistar大鼠根据体质量随机分为对照组(n=13)和实验组(n=29),对照组予普通饲料喂养,实验组予高脂饲料喂养。喂养6周后,每组各随机抽取5只,检测血脂。验证建模成功后,将实验组剩余大鼠(n=24)随机分为高脂对照组(n=8)、洛伐他汀组(n=8)、血脂康组(n=8),继续喂养高脂饲料,并给予洛伐他汀2.5 mg/(kg·d)、血脂康300.0 mg/(kg·d)干预。喂养12周后,检测各组大鼠血脂、肾功能、丙二醛(MDA)和总抗氧化能力(T-AOC);ELISA法检测血清肿瘤坏死因子α(TNF-α)和白细胞介素-6(IL-6);Masson、PAS染色及免疫组化方法测定TNF-α、转化生长因子-β(TGF-β)。结果高脂组大鼠甘油三酯(TG)、总胆固醇(TCHO)、低密度脂蛋白(LDL-C)、TNF-α、IL-6、血清肌酐(Scr)、血尿素氮(BUN)、血尿酸(UA)、MDA水平均高于药物干预组,差异均有统计学意义(P<0.05);高密度脂蛋白(HDL-C)、T-AOC水平低于药物干预组,差异均有统计学意义(P<0.05)。与高脂组比较,血脂康组和洛伐他汀组肾功能水平较低(P<0.05)。血脂康组的炎症水平低于洛伐他汀组(P<0.05),而抗氧化与洛伐他汀组相似(P>0.05);形态学检查发现,高脂组肾胶原增生。免疫组化提示,对照组TNF-α表达最少,高脂组肾小管上皮细胞TNF-α、TGF-β及肾小球系膜细胞TGF-β阳性表达最多,药物干预组其次。血脂康组和洛伐他汀组的血脂水平、肾功能、肾病理等方面差异均无统计学意义(P>0.05)。结论血脂康和洛伐他汀能够调节血脂,并通过降低TNF-α、IL-6、MDA,升高T-AOC水平,发挥抗炎、抗氧化作用,保护血脂异常引起的肾损伤。
Objective To investigate the effects of lipid-lowering on oxidative stress,inflammatory factor and kidney in hyperlipidemia rat models. Methods A total of 42 Wistar rats were randomly divided into the control group（ n = 13） and the experiment group（ n =29）. The rats in the control group were given normal feeding,the others in the experiment group were given high cholesterol diet feeding. After 6 weeks,5 rats in each group were randomly selected to detect the lipid. After the model establishment,the rest rats in the experiment group（ n = 24） were randomly divided into the hyperlipidemia model group（ n = 8）,lovastatin group（ n = 8） and the Hypocol group（ n = 8）. The rats in the Xuezhikang group were continuely fed with high cholesterol diet,and were given lovastatin（ 2. 5 mg/kg/day）and Xuezhikang（ 300. 0 mg/kg/day）. After 12 weeks,the serum lipid,renal function,malonaldehyde（ MDA） and total antioxidant capacity（ T-AOC） were detected. Tumor necrosis factor-α（ TNF-α） and interleukin-6（ IL-6） were detected by ELISA; TNF-α and transforming growth factor-β（ TGF-β） were detected by kidney Masson,PAS staining and immunohistochemistry. Results The levels of triglyceride（ TG）,TCHO,LDL-C,TNF-α,IL-6,Scr,BUN,UA and MDA in the hyperlipidemia model group were all higher than those of the drug intervention groups（ P〈0. 05）; the levels of HDL-C and T-AOC in the hyperlipidemia model group were lower than those of the drug intervention groups（ P〈0. 05）. Compared with the hyperlipidemia model group,the renal function level in the Xuezhikang group and the lovastatin group were lower（ P〈0. 05）. The level of inflammation in the Xuezhikang group was lower than that in the lovastatin group（ P〈0. 05）,while the antioxidant was similar to that in the lovastatin group（ P〉0. 05）. Morphological examination revealed that renal collagen proliferation in the hyperlipidemia model group. Immunohistochemical suggested that TNF-α was the lowest expression in t
作者
张子龙
黄樱硕
刘梅林
ZHANG Zi-long;HUANG Ying-shuo;LIU Mei-lin(Department of Cardiology, China Meitan General Hospital, Beijing 100028, China)
出处
《临床军医杂志》
CAS
2018年第5期571-576,共6页
Clinical Journal of Medical Officers
基金
"十二五"国家科技支撑计划课题(2012BAI37B05)
关键词
血脂异常
肾功能
炎症因子
氧化应激
调脂治疗
Hyperlipidemia
Kidney function
Inflammation factor
Oxidative stress
Lipid-lowering
