摘要
为探究百里精油抗氧化能力及其潜在的保肝作用,通过测定百里香精油对DPPH自由基、ABTS自由基的清除能力,确定百里香精油的体外抗氧化能力。同时建立硫代乙酰胺(TAA)急性小鼠肝损伤模型,通过测定小鼠的肝脏指数,血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)活性和肝脏中苯二醛(MDA)、谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)的活性来评价百里香精油的保肝效果。结果表明,百里香精油具有良好的体外抗氧化活性及对硫代乙酰胺诱导的肝损伤小鼠的保护效果,其中高剂量精油对小鼠肝损伤的保护效果显著(P<0.01)。本文旨在为百里香精油作为天然植物源保肝药物的研究与开发奠定理论基础。
The study was to explore the antioxidant capacity of thyme essential oil and its potential protection effects on the liver of mice. The anti-oxidation activities of thyme essential oil were analyzed by DPPH· and ABTS·＋scavenging experiments. Besides,the acute liver injury model of Kunming mice by Thioacetamide（ TAA） was also established,and the liver protection effects of thyme essential oil were assessed by determining the activity of ALT,AST and ALP in mice blood samples. After the homogenization of the liver tissue,the MDA,GSH contents and SOD,GSH-Px and CAT activities were further analyzed. The results suggested that thyme essential oil had significant anti-oxidation capacities and protection effects on the liver of the mice,especially when used at high doses. The study aimed to provide the theoretical foundation for the research and development of thyme essential oil.
作者
刘星
王锐
张润光
王静
王倩
张有林
LIU Xing;WANG Rui;ZHANG Run-guang;WANG Jing;WANG Qian;ZHANG You-lin(College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi＇ an, 710119, China;Shaanxi Provincial Forestry Technology Promotion Station, Xi＇ an, 710082, China;College of Life Science and Food Engineering , Xi＇ an , 710010, China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第5期856-862,共7页
Natural Product Research and Development
基金
陕西省科技攻关项目(2015NY007)
西安市科技计划(NC1405-2)
