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化痰活血扶正方及其拆方后的相关药组对模型大鼠肝纤维化指标的影响 被引量:1

Influence of phlegm reducing, blood activating, and body resistance strengthening decoction and its decomposed formulas on liver fibrosis in rats
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摘要 目的观察化痰活血扶正方拆方后的相关药组对肝纤维化大鼠模型肝功能及肝纤维化指标的影响,并探讨其作用机制.方法选择雄性SD大鼠120只,随机分为正常组(control)、模型组(model)、阳性药物组(Silybin)、低中高剂量化痰组(HTL、HTM、HTH)、低中高剂量活血化瘀组(HXHYL、HXHYM、HXHYH)、低中高剂量扶正组(FZL、FZM、FZH)、低中高剂量化痰活血扶正组(HTHXFZL、HTHXFZM、HTHXFZH).除正常照组外,其余各组大鼠均腹腔注射4%TAA(水为溶剂)0.05 mL/kg体重,剂量200 mg/kg,每周2次,持续8 wk.造模开始次日,各低、中、高剂量组分别按照0.25,0.5,1.0 g/kg剂量,每日1次、予10 m L/kg体重不同浓度的药物灌胃,阳性药物组给予水飞蓟宾50 mg/kg体重灌胃,每日1次,用药8 wk.观察治疗前后的肝功能[谷草转氨酶(aspartate transaminase,AST)、谷丙转氨酶(alanine transaminase,ALT)、总蛋白(total protein,TP)、AIb、GLB]、肝纤维化指标[层黏连蛋白(laminin,LN)、COL_4、透明质酸(hyaluronic acid,HA)].结果干预后,各组血清的AST、ALT均有下降,除FZL组外,其余各组与模型组相比差异均有统计学意义(P<0.05、P<0.01或P<0.001);HTM、HTHXFZH的TP与模型组相比差异有统计学意义(P<0.05或P<0.01),HTM、HTH、HXHYM、FZM、FZH、HTHXFZM、HTHXFZH的AIb与模型组相比差异均有统计学意义(P<0.05、P<0.01或P<0.001).各治疗组的LN均有下降,除HXHYM组、FZM组外,其余各组与模型组相比差异显著,均有统计学意义(P<0.05、P<0.01或P<0.001);各治疗组的COL_4均有下降,除HTL、HTH、HXHYM、HXHYH、FZL、FZH组外,其余各组与模型组相比差异显著,均有统计学意义(P<0.05或P<0.001);各治疗组的HA均有下降,其中HXHYM、FZL、HTHXFZH与模型组相比差异显著,均有统计学意义(P<0.05或P<0.01).结论化痰活血扶正方拆方后各药组可显著改善肝纤维化模型大鼠的肝功能及肝纤维化指标,减缓肝纤维化进展速度. AIM To observe the effect of phlegm reducing, blood activating, and body resistance strengthening decoction and its decomposed formulas on liver function and hepatic fibrosis indexes in rats with liver fibrosis, and to explore the underlying mechanism.METHODS One hundred and twenty male SD rats were randomly divided into a normal group, a model group, a positive drug(silybin) group, low-, middle-, and high-dose phlegm reducing groups(HTL, HTM, and HTH), low-, middle-, and high-dose blood activating groups(HXHYL, HXHYM, and HXHYH), low-, middle-, and high-dose body resistance strengthening groups(FZL, FZM, and FZH), and low-, middle-, and high-dose phlegm reducing, blood activating, and body resistance strengthening decoction groups(HTHXFZL, HTHXFZM, and HTHXFZH). Except the normal group, the rats in the other groups were injected with 4% TAA in water at a dose of 200 mg/kg, twice a week for 8 wk. The next day after modelling, the low-, middle-, and high-dose groups were intragastrically given corresponding drugs at 0.25, 0.5, and 1.0 g/kg(once daily), respectively, and the positive drug group was given silybin 50 mg/kg body weight once daily for 8 wk. The liver function [aspartate transaminase(AST), alanine transaminase(ALT), total protein(TP), Alb, and GLB] and hepatic fibrosis indexes [laminin, COL4, hyaluronic acid(HA)] were observed before and after treatment.RESULTS After intervention, serum AST and ALT decreased in all groups except the FZL group, and the differences between the other groups and the model group were statistically significant(P〈0.05, P〈0.01, or P〈0.001). Serum TP in the HTM and HTHXFZH groups was significantly different from that in the model group(P〈0.05 or P〈0.01). Serum Alb in the HTM, HTH, HXHYM, FZM, FZH, HTHXFZM, and HTHXFZH groups was significantly different from that in the model group(P〈0.05, P〈0.01, or P〈0.001). Serum LN decreased in each treatment group, and except the HXHYM and FZM groups, the difference between
作者 林唐唐 郑保平 刘海华 唐杨 肖海 韩立民 Tang-Tang Lin;Bao-Ping Zheng;Hai-Hua Liu;Yang Tang;Hai Xiao;Li-Min Han(Department of Traditional Chinese Medicine, the First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, Jiangxi Province, China;Department of Pathology, the First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, Jiangxi Province, Chin;Headmaster's Office of Gannan Medical University, Ganzhou 341000, Jiangxi Province, China)
出处 《世界华人消化杂志》 CAS 2018年第18期1095-1101,共7页 World Chinese Journal of Digestology
基金 国家自然科学基金 No.81460707 江西省教育厅科学技术研究项目基金 No.14686~~
关键词 肝纤维化 化痰法 化痰活血扶正方 拆方 Liver fibrosis Reducing phlegm Phlegmreducing Blood activating Body resistance strengtheningdecoction Decomposed formulas
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