摘要
目的:探讨虾青素对凹透镜所致的豚鼠近视模型屈光状态及病理组织形态的影响,阐明虾青素对近视屈光状态的改善作用。方法:48只健康豚鼠随机分为空白对照组(n=12)和模型组(n=36),模型组以远视性光学离焦法制备豚鼠右眼近视模型(左眼作为对照)后随机分为模型对照组、低(25ig·kg^(-1))和高剂量虾青素组(50ig·kg^(-1)),给药干预4周,于2和4周分别检测各组豚鼠双眼屈光度和眼轴长度,并于4周检测完成后,对右眼进行病理组织形态学检查。结果:模型组豚鼠在实验2周后实验眼球屈光度较作为对照的左眼低(P<0.05),与空白对照组右眼比较也明显降低(P<0.05),4周后更加明显(P<0.01);模型组豚鼠在实验2周后实验眼球的眼轴长度较对照的左眼及空白对照组增加(P<0.05),4周后增加更为明显(P<0.01)。与模型组比较,虾青素组豚鼠眼屈光度及眼轴长度明显增加(P<0.05),用药时间长且效果较好;其病理组织形态学上也呈现出明显的改善,胶原断裂现象明显减少且排列较整齐。结论:虾青素在改善近视眼屈光度及眼轴长度方面有良好的效果,还有助于恢复近视眼球巩膜的组织形态。
Objective：To investigate the effect of astaxanthin on the refractive status and pathological morphology of the guinea pig myopia models induced by concave lens,and to elucidate the improvement effect of astaxanthin on the refractive status of myopia.Methods：A total of 48 healthy guinea pigs were randomly divided into blank control group（n=12）and model group（n=36）;the guinea pig myopia models（right eyes）were established with far vision optical method（left eyes were used as control）.The guinea pig models were divided into model control group,high of astaxanthin（50ig·kg^-1）group and low dose of astaxanthin（25ig·kg^-1）group.After 2 and 4 weeks treatment,the diopters and the lengths of optic axis of both eyes of each guinea pig in various groups were tested,and at 4 weeks the right eyes of the pigs were removed and the pathomorphology was observed by HE staining.Results：The diopters of the model eyes in model group were lower than those of left eyes used as control（P〈0.05）and those of right eyes in blank control group after 2 and 4 weeks（P〈0.05 or P〈0.01）.The length of optic axis of the model eye in model group was increased compared with left eye used as controls and blank control group after 2 weeks（P〈0.05）and especially at 4 weeks（P〈0.01）.Compared with model group,the axial oculi and diopters of the guinea pigs in astaxanthin groups were increased（P〈0.05）;the pathohistological changes were improved,the collagen fracture was obviously decreased,and the collagen fibers were orderly arranged.Conclusion：Astaxanthin can recover the length of optic axis,diopter and histomorphology of sclera of guinea pig myopia models in some extents.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2017年第5期932-936,I0005,共6页
Journal of Jilin University：Medicine Edition
基金
河北省科技厅自然科学基金资助课题(H2015406054)
河北省承德市科技局自筹经费项目资助课题(201121058)
关键词
虾青素
近视
屈光度
眼轴长度
豚鼠
疾病模型
动物
astaxanthin
myopia
diopter
length of optic axis
guinea pig
disease model, animanl
