摘要
分别采用气相色谱-质谱联用法、超高效液相色谱串联三重四极杆质谱法、紫外可见分光光度法、粗纤维测定法、BCA试剂盒法对黄蜀葵[Abelmoschus manihot(L.)Medic.]种子中的主要成分进行分析评价。结果表明,黄蜀葵种子含有丰富的脂肪酸类、可溶性总多糖、总纤维、可溶性蛋白、游离氨基酸类、核苷及碱基类成分,脂肪酸总量(棕榈油酸、棕榈酸、亚油酸、油酸、亚麻酸、硬脂酸)为55.47~102.17 mg/g,不饱和脂肪酸占脂肪酸总量的78.01%~79.40%;可溶性总多糖、总纤维和可溶性蛋白含量分别为6.53%~6.68%、12.77%~14.26%、10.36%~14.51%;检出的19种游离氨基酸类和7种核苷及碱基类成分中,氨基酸类成分较为丰富100.82~101.51 mg/g,其中必需氨基酸约占游离氨基酸总量的25%;核苷类成分含量较低(3.01~3.11 mg/g)。研究结果为黄蜀葵种子的资源化利用提供了理论依据。
The chemical constituents of the seeds of Abelmoschus manihot（ L.） Medic. were determined by GC-MS,UV-Vis spectrophotpmetry,Weende,BCA and UPLC-TQ/MS. The seed of A. manihot was rich in fatty acids,soluble polysaccharides,celluloses,soluble protein,amino acids and nucleosides.Total content of the fatty acids（ palmitoleic acid,palmitic acid,linolic acid,oleic acid,linolenic acid,stearic acid） was 55.47 -102.17 mg/g,and the unsaturated fatty acids accounted for 78.01% -79.40% of all these fatty acids. The contents of soluble polysaccharides,celluloses and soluble protein were 6.53% - 6.68%,12.77% - 14.26%,and 10.36% -14.51%,respectively. Total of 19 amino acids and 7 nucleosides were detected in the seed,and the content of amino acids were 100.82 -101.51 mg/g,in which essential amino acids were accounted for 25%.The content of nucleosides in the seed was determined as 3.01 - 3.11 mg/g. The results provided a theoretical basis for the resourceful utilization of the seed of A.manihot.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第14期20-25,39,共7页
Science and Technology of Food Industry
基金
江苏省产学研合作前瞻性联合研究项目(BY2015008-04)
江苏省高校中药学优势学科II期建设项目(2014-ysxk)
关键词
黄蜀葵
种子
化学成分
资源化利用
Abelmoschus manihot（L.） Medic
seed
chemical composition
resourceful utilization
