摘要
该研究应用Illumina Hiseq 2500高通量测序平台,分析了未种植黄连土壤、黄连健株及病株根际土壤的细菌种群丰富度及多样性变化,并结合土壤养分及酶活变化检测,对黄连根腐病产生的机制进行了深入探讨。高通量测序显示,栽培黄连造成了土壤细菌种群的丰富度的显著降低(P<0.05)和细菌种群多样性的降低;罹患根腐病的黄连植株根际土壤细菌的种群丰富度要显著低于未种植和健康黄连土(P<0.05),种群多样性要显著低于未种植黄连土样(P<0.05),但与健株土样之间差异不显著。对土壤的养分及酶活测定结果表明,栽培黄连造成了土壤pH、有效磷、脲酶活性的显著降低,蔗糖酶活性的显著升高(P<0.05);根腐病土样中有机碳的含量、碱解氮、过氧化氢酶和脲酶活性显著低于健康土样,速效磷和速效钾含量显著高于健康土样(P<0.05)。综合分析表明,栽培黄连造成了土壤中细菌种群丰富度和多样性的降低;病株土样中,细菌种群丰富度的显著降低和种群多样性的降低,有可能是导致黄连根腐病产生的一个重要原因,此外,土壤有机碳和过氧化氢酶活性的显著减低,也许是导致黄连根腐病发生的一个诱因。
Illumina Hiseq 2500 high-throughput sequencing platform was used to study the bacteria richness and diversity, the soil enzyme activities, nutrients in unplanted soil, root-rot and healthy rhizophere soil of Coptis chinensis for deeply discussing the mechanism of the root-rot of C. chinensis. The high-throughput sequencing result showed that the artificial cultivation effected the bacteria community richness and diversity. The bacteria community richness in healthy and diseased rhizosphere soil showed significant lower than that of in unplanted soil （P〈0.05） and declined bacteria diversity. The bacteria community richness in root-rot rhizosphere soil increased significantly than that of health and unplanted soil and the diversity was lower significant than that of unplanted soil （P〈0.05）. The results of soil nutrients and enzyme activities detected that the pH value, available phosphorus and urease activity decreased and the sucrase activity increased significantly （P〈0.05）. The content of organic carbon and alkaline hydrolysis nitrogen the catalase and urease activity in root rot soil samples was significantly lower than that of healthy soil samples （P〈0.05）. However, the contents of available phosphorus and available potassium were significantly in root-rot sample higher than that of healthy soil samples （P〈0.05）. Comprehensive analysis showed that the artificial cultivation declined the bacteria community richness and diversity. The bacteria community richness decreased significantly and the decreased diversity may be the cause of the root-rot. Meanwhile, the decrease of carbon and the catalase activity may be another cause of the root-rot in C. chinensis produced in Shizhu city, Chongqing province.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第7期1304-1311,共8页
China Journal of Chinese Materia Medica
基金
国家"十二五"科技支撑计划项目(2011BAI13B02-1)
国家科技基础性工作专项(2015FY11500-120)
重庆市平台与基地建设项目(cstc2014ptjd10001)
重庆市基本科研业务项目(2015cstc-jbky-01904)
关键词
黄连
根腐病
土壤酶活性
土壤养分
高通量测序
细菌
Coptis chinensis
root-rot
enzyme activities in soil
the nutrients of soil
illumina high-throughput sequencing
bacteria
