摘要
【目的】以苎麻(Boehmeria nivea L.Gaud)根及根围土壤为研究材料,进行苎麻促生菌的筛选,并初步探索其促生作用机制。【方法】首先,以溶磷和解钾为基本筛选标准,初筛菌株在实验室条件下测定多项促生能力进行复筛;然后通过种子萌发、盆栽试验测定菌株对苎麻的促生效应,最后,通过形态观察、生理生化特性和16S rRNA基因序列同源性分析,对促生菌株进行分类学鉴定。【结果】从苎麻根和根围土壤中分离得到了13株菌同时具备溶磷和解钾能力,其中4株菌(RA-2、RAM-2、RAM-5和RAM-6)具备产铁载体、产IAA和产氨能力。种子萌发和盆栽试验的测定结果显示:4株菌株均能促进苎麻种子的萌发和植株的生长,其中菌株RA-2和RAM-5相比于对照处理能显著提高苎麻种子的萌发率、幼根长、株高和根系干重。分类鉴定结果显示菌株RA-2和RAM-5均属于伯克霍德菌属(Burkholderia)。【结论】从苎麻根围筛选到具有促生能力的菌株,为进一步开发研制苎麻专型促生菌剂或专型微生物有机肥提供资源。
[Objective] We used roots and rhizosphere soils of ramie（Boehmeria nivea L.Gaud） to isolate plant growth promoting bacteria（PGPB）,and then studied the mechanism why they promote growth of ramie in preliminarily.[Methods] Preliminary screening of PGPBs under the premises of bacteria have the abilities of phosphate-and potassium-solubilizing,moreover,the bacteria strains were screened by testing their abilities of siderophore,indole acetic acid（IAA） and ammonia production in vitro.Then,seeds germination and pot experiments were conducted to measure the promoting-growth effects on ramie of the isolates.Finally,PGPBs were classified and identified by combining physiological and biochemical tests and 16 S rRNA gene sequences analysis.[Results] Thirteen strains were isolated based on the abilities of phosphate- and potassium-solubilizing,of which four strains（RA-2,RAM-2,RAM-5,and RAM-6） also exhibited plant growth promoting properties like siderophores,IAA and ammonia production.Seeds germination and pot experiments showed that four selected strains could promote growth of ramie,moreover,strain RA-2 and RAM-5 could significantly improve the germination rates,root lengths,plant heights,and root dry weights,both of isolates RA-2and RAM-5 were identified as Burkholderia sp..[Conclusion] PGPBs which were isolated from ramie rhizosphere will helpful for developing the specially PGPB microbial inoculants or bio-organic fertilizers of ramie.
出处
《微生物学通报》
CAS
CSCD
北大核心
2015年第3期525-533,共9页
Microbiology China
基金
国家麻类产业技术体系项目(No.CARS-19-08B)
关键词
植物促生细菌
苎麻
生长发育
伯克霍尔德氏菌
Plant growth-promoting bacteria
Ramie
Growth and development
Burkholderia sp.
