摘要
【目的】揭示黄连根腐病与根部可培养真菌及根际土壤真菌组成的关系。【方法】用高通量测序法测定健康植株和根腐病植株根际土壤的真菌组成,并用组织分离法分离鉴定其根部真菌。【结果】病株和健株根际土壤真菌多样性、种属没有显著性差异。健株根和病株根中分离出的真菌组成有明显差别:病株根中优势种是Phoma herbarum;优势属是Phoma sp.、Ilyonectria sp.。健株根中优势种是DSE真菌Cadophora orchidicola;优势属是Cadophora sp.。黄连根部可培养真菌的Shannon-Wiener指数种水平上病株均比健株高,属水平上健株比病株高。【结论】土壤中真菌数量及组成对根腐病的发生没有显著影响;Ilyonectria sp.可能包含病原真菌。根部真菌属水平多样性的降低可能与根腐病发生相关。
【Objective】The present paper aimed to disclose the relationship between rhizosphere soil,root fungi composition and root rot disease of C.chinensis.【Method】The fungi composition of rhizosphere soil was analyzed by Illumina high-throughput Sequencing and the fungi of healthy and rot roots were isolated and identified.【Result】There was no significant difference between the fungi diversity,OTUs and abundance of healthy and root rot individual rhizosphere soil.There was obvious difference between the fungi composition of healthy and rot root.Phoma herbarum,Phoma sp.and Ilyonectria sp.were dominant in rot root while Cadophora orchidicola and Cadophora sp.were dominant in healthy one.The Shannon-Wiener index of cultivable fungi in rot root was higher than that of healthy one at species level,while lower at genus level.【Conclusion】The fungi composition of rhizosphere soil had no significant effect on root rot disease.Ilyonectria sp.may contain pathogen.The decrease of fungi diversity at genus level in C.chinensis root may have relation with root rot disease.
作者
伍晓丽
李隆云
王钰
刘飞
陈大霞
WU Xiao-li;LI Long-yun;WANG Yu;LIU Fei;CHEN Da-xia(Institute of Material Medical Planting,Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China;Chongqing Subcenter of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Science,Chongqing 400065,China;Health Central,Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)
出处
《西南农业学报》
CSCD
北大核心
2020年第11期2509-2518,共10页
Southwest China Journal of Agricultural Sciences
基金
基本科研业务费项目“木霉对石柱黄连根腐病的防治机理研究”(cstc2019jxjl-jbky10004)
重庆市卫计委重点项目“木霉类生防真菌对黄连根腐病的防治研究”(ZY201801006)
重庆市自然基金“基于大数据分析的DSE菌与黄连根腐病发生的相关性研究”(cstc2019jcyj-msxmX0677)
国家中药材产业技术体系(CARS-21)。
关键词
黄连根腐病
根部可培养真菌
Illumina高通量测序
根际土壤真菌组成
DSE菌
Root rot disease of Coptis chinensis
Root cultivable fungi
Illumina high-throughput sequencing
Rhizosphere soil fungi composition
DSE fungi
