摘要
背景:获取高纯度、高活性的成骨细胞是进行骨代谢研究的基础。目的:探索一种简便、高效的原代成骨细胞培养方法。方法:将新生24 h内的SD大鼠,分离获取颅骨的顶骨和额骨,预先采取胰酶和胶原酶消化,再进行组织块培养的方法提取成骨细胞。通过倒置相差显微镜和透射电镜观察细胞形态,细胞计数绘制细胞生长曲线,采用碱性磷酸酶BCIP/NBT染色和茜素红矿化结节染色进行成骨细胞鉴定。结果与结论:(1)细胞形态呈长梭形、三角形、不规则多边形,有两至三个细胞突起;(2)透射电镜下线粒体和内质网在成骨细胞中非常丰富,并且有较多细胞突起,呈现出典型成骨细胞特点;(3)细胞刚接种时生长缓慢,3 d后增殖加快,第7天达高峰;(4)碱性磷酸酶BCIP/NBT染色显著阳性,茜素红钙结节染色橘红色;(5)采用酶消化法联合组织块法提取的细胞具有典型的成骨细胞特征和功能,是一种理想的原代成骨细胞分离培养方法。
BACKGROUND：Osteoblasts with high purity and activity are essential for bone metabolism research. OBJECTIVE：To explore a simple and effective culturing method of primary osteoblasts. METHODS：Osteoblasts were isolated from the parietal and frontal bones of newborn Sprague-Dawley rats using trypsin and collagenase digestion and tissue explant method. The morphology of osteoblasts was observed by inverted phase contrast microscope and transmission electron microscope;the cells was counted to draw the growth curve;the osteoblasts were identified by alkaline phosphatase BCIP/NBT staining and alizarin red staining. RESULTS AND CONCLUSION：The cells showed spindle, triangle or polygon shapes, having two or three protrusions. There were abundant mitochondria and endoplasmic reticulum under electron microscope, which presented the typical characteristics of osteoblasts. The cell growth was slow intially, accelerating at the 3rd day, and peaking at the 7th day. The cells were highly positive for alkaline phosphatase staining and were stained orangered through the alizarin red staining. To conclude, the cells isolated using enzymatic digestion combined with tissue explant method exhibit the typical characteristics and functions of osteoblasts, and this method is an ideal way to culture primary osteoblasts.
出处
《中国组织工程研究》
CAS
北大核心
2017年第12期1833-1837,共5页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(81260142)~~
