摘要
目的:探讨葡萄糖对体外培养的新生大鼠下颌骨成骨细胞(new born ratmandibular osteoblasts,NRMOBs)增殖的影响。方法:取新生6-7d(Sprague Dawley,SD)乳鼠下颌骨,原代培养下颌骨成骨细胞,选取生长状态良好的第4代细胞,在糖生理浓度(5.5mM)和高糖条件下(16.5mM和49.5mM)培养,于1,3,5,7,9d采用MTT(Methylthiazol Tetrazolium,MTT)法检测不同葡萄糖浓度对体外培养的NRMOBs增殖的影响。采用SPSS13.0软件方差分析对数据进行统计。结果:所培养的NRMOBs阳性表达波形丝蛋白、骨钙素,角蛋白表达阴性,分泌碱性磷酸酶,形成钙结节。细胞在高糖培养1d时,16.5m M与49.5m M葡萄糖均下调了细胞的增殖,49.5m M葡萄糖的抑制有显著性差异(P<0.05);3d时,16.5mM与49.5m M葡萄糖均开始促进细胞的增殖,但与5.5m M葡萄糖比没有显著性差异;细胞培养5、7、9d时,16.5mM葡萄糖显著促进了细胞的增殖(P<0.05),49.5m M葡萄糖在9d时也显著促进了细胞的增殖(P<0.05)。结论:葡萄糖浓度影响NRMOBs的增殖。细胞培养早期,高糖抑制NRMOBs的增殖;细胞培养晚期,高糖逐渐促进NRMOBs的增殖。
Objective： To investigate the effect of glucose on the proliferation of newborn rat mandibular osteoblasts（NRMOBs）.Methods： The NRMOBs were cultured in vitro.The effect of different doses of glucose concentration（5.5、 16.5 and 49.5mmol/L） on NRMOBs was investigated.The NRMOBs proliferation was examined after 1、 3、 5、 7、 9 days of culture using Methylthiazol Tetrazolium（MTT）.Results： The NRMOBs positively expressed vimentin and osteocalcin,negatively expressed keratin,secreted alkaline phosphatase（ALP）,and produced calcium nodules.At 1th day of culture,16.5mM glucose decreased slightly NRMOBs proliferation,and 49.5mM glucose significantly decreased NRMOBs proliferation.The 16.5mM and 49.5mM glucose started to increase NRMOBs proliferation at 3th day of culture,compared to 5.5mM glucose group,the differences had no significance.At 5th、7th、9th day of culture,16.5mM glucose significantly increased NRMOBs proliferation.The 49.5mM glucose started significantly to increase NRMOBs proliferation at 9th day of culture.Conclusion： This study suggests that glucose concentration affects NRMOBs proliferation.Early stage,high-glucose inhibits the proliferation of NRMOBs.Later period,high-glucose gradually promotes the proliferation of NRMOBs.
出处
《中华老年口腔医学杂志》
2012年第6期321-324,共4页
Chinese Journal of Geriatric Dentistry
