摘要
建立酒花黄酮的提取方法和含量测定方法。采用NaNO2-Al(NO3)3-NaOH显色法,以芦丁为对照品,通过单因素试验和L9(34)正交设计确定酒花黄酮的提取方法,并测定和比较酒花、酒糟和啤酒中黄酮的含量。结果表明:酒花黄酮提取方法为样品加入50倍的甲醇,超声提取2次,每次1h,酒花黄酮含量为69.98mg/g,酒糟和啤酒中黄酮的含量较低。对照品芦丁在10.2～40.8μg/mL质量浓度范围内与吸光度呈良好线性关系,平均回收率99.33%,RSD=3.60%。该提取方法合理,含量测定方法简便、快速、准确,可用于酒花黄酮提取物制备及其质量控制。
Ultrasonic extraction conditions for total flavonoids from Humulus lupulus L.were optimized by single factor experiments and orthogonal design with the aim of establishing a sample preparation method for determination of the content of total flavonoids in Humulus lupulus L.by NaNO2-Al（NO3）3-NaOH colorimetric method using rutin as the reference.The optimal extraction process parameters were material-to-liquid ratio of 1：50,extraction duration of 1 h and repeated extraction number of 2.The content of total flavonoids from Humulus lupulus L.was 69.98 mg/g under the optimal extraction conditions,which was much higher than that of residues and beer.The linear range was between 10.2μg/mL and 40.8μg/mL with a correlation coefficient of 0.9985.The average recovery rate was 99.33% with a relative standard deviation of 3.60%.The extraction and determination methods were simple and feasible,which is suitable for the preparation and quality control of flavonoids extracted from Humulus lupulus L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第6期16-19,共4页
Food Science
基金
新疆维吾尔自治区高校科研计划重点项目(XJEDU2009I24)
关键词
酒花
黄酮
提取工艺
含量测定
Humulus lupulus L.
total flavonoids
extraction
determination
