摘要
目的探讨白细胞介素-17A(IL-17A)、白细胞介素-17F(IL-17F)基因多态性与接尘工人肺部炎症遗传易感性的相关性。方法选择67例肺部炎症患者作为病例组,126例相同粉尘暴露的健康工人作为对照组,现场监测其粉尘暴露情况;调查其人口学特征、职业信息等;采集外周静脉血,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析和验证IL-17A(G-197A)和IL-17F(7488T/C)两位点基因多态性,对IL-17A、IL-17F基因多态性与接尘工人肺部炎症易感性进行logistic回归分析。结果 1193例接尘工人中IL-17A(G-197A)基因型A/A、A/G、G/G分别有42例(21.76%)、95例(49.22%)、56例(29.02%);IL-17F(7488T/C)基因型T/T、T/C、C/C分别有128例(66.32%)、54例(28.98%)、11例(5.70%);各基因型的分布频率符合遗传学的HardyWeinberg平衡。2病例组IL-17A(G-197A)A/A基因型比例低于对照组(P<0.05),而A/G、G/G基因型比例均高于对照组(P<0.05);经年龄、吸烟情况、接尘浓度调整后,IL-17A(G-197A)A/G基因型(OR=5.03,P<0.01)、G/G基因型(OR=3.35,P<0.05)是接尘工人患肺部炎症的易感因素。3IL-17F(7488T/C)各基因型在病例组、对照组间分布差异无统计学意义。结论在相同的粉尘暴露条件下,与IL-17A(G-197A)A/A基因型相比,A/G、G/G基因型个体具有更高的患肺部炎症的风险,提示IL-17A(G-197A)A/G、G/G基因型的接尘个体可能对肺部炎症更具有易感性。
Objective To investigate the polymorphisms in interleukin 17A IL-17A） and interleukin 17F （IL-17F） and their relationship with pulmonary inflammation risk of dust exposed workers. Methods A case-control study among 193 subjects, including 67 subjects in case group and 126 in control group was conducted. PCR-RFLP was applied to genotype IL-17A （G-197A） and IL-17F （7488T/C）. Logistic regression analysis was used to determine the effects of IL-17A （G-197A） and IL-17F （7488T/C） on the lung inflammation risk in dust exposed workers. Results The genotypes analysis showed that the proportions of IL-17A （G-197A） A/A, A/G and G/G were 42 （21.76%）, 95 （49.22%）, 56 （29.02%） in 193 cases, respectively, and the IL-17F （7488T/C） T/T, T/C and C/C genotypes were 128 （66.32%）, 54 （28.98%）, 11 （5.70%）, respectively. The frequency distribution of each genotype was consistent with the Hardy-Weinberg equilibrium fixed law. The ratio of IL-17A （G-197A） A/A in the case group was lower than that of control group （ P〈0.05）, while the G/G and A/G genotypes were higher than that of control group （ P〈0.05）. Furthermore, the genotypes of IL-17A （G-197A） A/G （OR=5.03, P〈0.01） and G/G（OR=3.35, P〈0.05） were associated with an increased risk of lung inflammation in workers exposed to dust. The frequency distribution difference of IL-17F （7488T/C） T/T, T/C and C/C genotypes in the cases and control group was unobvious （ P〉0.05）. Conclusion Under the same dust concentration, the dust exposed workers carrying IL-17A （G-197A） A/G, G/G genotypes are more susceptible to pulmonary inflammation in the southwest of China.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2017年第1期86-90,共5页
Journal of Sichuan University(Medical Sciences)
