摘要
目的 探讨白细胞介素17(IL-17)rs4711998、rs763780基因多态性与尘肺易感性的关系,为尘肺病高危人群的预防提供依据.方法 选择219例尘肺病患者和242例接尘者为研究对象,拍摄X射线高千伏后前位胸片,按尘肺病诊断标准进行诊断;采集研究对象外周静脉血3 ml,应用PCR-RFLP技术检测IL-17基因多态性.结果 IL-17 A的rs4711998位点分为AA、AG和GG基因型,3种基因型在两组研究对象中的分布差异无统计学意义(P>0.05).IL-17 F的rs763780位点分为AA、AG和GG基因型,3种基因型在两组中的分布差异有统计学意义(P﹤0.05),等位基因G、等位基因A在两组的差异均有统计学意义(P﹤0.05).结论 IL-17A rs4711998位点基因多态性可能与尘肺病的易感性不相关;IL-17F rs763780位点基因多态性可能与尘肺病的易感性相关,尘肺的保护基因型是AG,尘肺的保护等位基因是G等位基因.
Objective To explore the relationship between polymorphisms of interleukin 17 (IL-17) gene (rs4711998, rs763780) and the susceptibility of pneumoconiosis, and to provide a basis for prevention of high-risk groups of pneumoconiosis. Methods A total of 219 pneumoconiosis patients and 242 workers without pneumoconiosis were enrolled in the study. All subjects were photographed with high undulating X-rays anterior chest radiographs, diagnosed according to diagnostic criteria for pneumoconiosis. We collected 3 ml of peripheral venous blood of the study subjects. Polymorphism in IL-17A rs4711998 and IL-17F rs763780 locus were evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The IL-17A r.s4711998 locus has AA, AG and GG genotypes, there was no the significant difference between case and control groups (P>0.05). IL-17F rs763780 had AA, AG and GG genotypes, there was a significant difference between case and control groups (P <0.05). Allele A and allele G were statistically significant difference between the case group and the control group (P<0.05). Conclusion No relationship was found be tween IL-17A gene polymorphisms at rs4711998 and silicosis. IL-17F rs763780 locus gene polymorphism is as sociated with susceptibility to pneumoconiosis. AG genotype and G allele may have a protective effect.
作者
张政
陈颖
韩科
王怀宾
王苗苗
肖坤
沈福海
Zhang Zheng;Chen Ying;Han Ke;Wang Huaibin;Wang Miaomiao;Xiao Kun;Shen Fuhai(School of Public Health, North China University of Science and Technology, Tangs han 063210, China)
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2019年第6期416-419,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
河北省自然科学基金项目(H2017209195).
