摘要
目的探讨儿童IL-17A启动子区域（-197G/A和-692C/T）基因多态性与儿童哮喘易感性的关系,为能进一步寻找到哮喘的候选基因从而为患病高风险儿童早期预防奠定基础。方法选取2013年8月至2015年8月门诊随访或住院的哮喘患儿65例为哮喘组,另选取同期行健康体检儿童70例为健康对照组,采集两组儿童外周静脉血,应用序列特异性引物聚合酶链反应（SSP-PCR）法检测IL-17A基因-197G/A和-692C/T两个位点的单核苷酸多态性（SNP）,统计分析两组间基因型及等位基因分布频率的差异。结果 IL-17A基因-692C/T位点哮喘组患儿TT基因型的分布频率（29%）显著高于健康对照组（16%）（P=0.012）;哮喘组-692T等位基因分布频率（52%）显著高于健康对照组（42%）（P=0.039）;罹患儿童哮喘的风险T等位基因携带者是C等位基因携带者的1.413倍（OR=1.413,95%CI：1.015~1.917）;而IL-17A基因-197G/A位点基因型及等位基因分布频率在哮喘组和健康对照组间比较差异无统计学意义（P〉0.05）。结论 IL-17A基因启动子区域-692C/T位点基因多态性与儿童哮喘的易感性相关,-692T等位基因携带者更易罹患儿童哮喘,而IL-17A-197G/A位点多态性与儿童哮喘的易感性无显著相关。
Objective To explore the relationship between polymorphisms of interleukin-17A（IL-17A） gene promoter（-197G/A and-692C/T） and the susceptibility to childhood asthma,to further identify the candidate genes for asthma,and to provide a basis for early prevention of asthma in high-risk children.Methods Sixty-five outpatients or inpatients with childhood asthma between August 2013 and August 2015 were assigned to asthma group.Seventy healthy children within the same period were assigned to control group.Using peripheral venous blood from the two groups,PCR with sequence-specific primers was carried out to determine single nucleotide polymorphisms at positions-197G/A and-692C/T in IL-17 A gene promoter.A statistical analysis was used to evaluate differences in genotype and allele frequencies between the two groups.Results Compared with the control group,the asthma group had significantly higher frequencies of TT genotype（29% vs 16%; P=0.012） and T allele（52% vs 42%; P=0.039） at position-692C/T of IL-17 A gene.Children with T allele had 1.413-fold higher risk of childhood asthma than those with C allele（OR=1.413,95%CI： 1.015-1.917）.There were no significant differences in genotype and allele frequencies at position-197G/A in IL-17 A gene between the two groups（P〈0.05）.Conclusions Polymorphisms at position-692C/T in IL-17 A gene promoter is associated with the susceptibility to childhood asthma.Children with-692 T allele are more susceptible to childhood asthma.There is no significant relationship between polymorphisms at position-197G/A in IL-17 A gene promoter and the susceptibility to childhood asthma.
作者
钟芳芳
邹艳
刘春艳
刘文君
ZHONG Fang-Fang ZOU Yan LIU Chun-Yan LIU Wen-Jun.(Department of Pediatrics, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, Chin)
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2016年第12期1264-1268,共5页
Chinese Journal of Contemporary Pediatrics
基金
西南医科大学附属医院科研基金(2015-QS-032)
关键词
IL-17A
哮喘
易感性
单核苷酸多态性
儿童
Interleukin-17A
Asthma
Susceptibility
Single nucleotide polymorphism
Child
