摘要
[目的]探讨去甲斑蝥素对人胃癌SGC-7901细胞抑制和诱导凋亡作用。[方法]采用不同浓度的去甲斑蝥素(noncantharidin,NCTD)作用于胃癌SGC-7901细胞,在扫描电镜下观察其形态特点,采用流式细胞术测定经NCTD作用后的细胞生长周期及凋亡率,Western-blot检测Bcl-2、Mcl-1、Bax的表达。[结果]扫描电镜下可见,SGC-7901细胞出现凋亡形态学改变。经去甲斑蝥素处理SGC-7901细胞后,实验组G2/M细胞明显多于对照组(P<0.05)。10μg/ml和20μg/ml浓度去甲斑蝥素均能诱导胃癌SGC-7901细胞凋亡(P<0.05),其抑制率与NCTD浓度及作用时间相关。Western-blot检测Bcl-2、Mcl-1蛋白表达减少,Bax蛋白表达增加,呈明显的剂量关系。[结论]去甲斑蝥素对人胃癌SGC-7901细胞有抑制作用,可能与其阻滞细胞周期诱导细胞凋亡有关。
[Purpose] To explore inhibition and apoptosis of norcantharidin on human gastric cancer SGC-7901 cell. [Methods] Gastric cancer SGC-7901 cells were treated with different concentrations（5,10,20,40μg/ml） of norcantharidin（NCTD） for 24,48,72 h in vitro, morphological characteristics of apoptosis were observed with elelectron microscope,the cell growth cycle and apoptosis rate were determined by flow cytometry. Bcl-2,Mcl-1 and Bax expression were detected with Western blot. [Results] Under the electron microscope,SGC-7901 cells appeared morphological changes of apoptosis. After SGC-7901 treated with norcantharidin,G2/M cells in the experimental group were significantly higher than that in control group（P0.05）. The 10μg/ml and 20μg/ml norcantharidin induced apoptosis of gastric cancer SGC-7901 cells（P0.05）, in a concentration and timedependeit manner. The expression of Bcl-2 and Mcl-1 protein expression was decreased,the Bax protein expression increased in a dose-dependent manner. [Conclusion] Norcantharidin has an inhibitory effect on human gastric cancer SGC-7901 cells,which is related to cell cycle arrest and apoptosis.
出处
《中国肿瘤》
CAS
2016年第12期999-1003,共5页
China Cancer
基金
河北中医学院青年科研基金(QNZ2015010)
关键词
去甲斑蝥素
胃癌
细胞周期
细胞形态
norcantharidin
gastric cancer
cell cycle
cell morphology
