摘要
目的:通过对比研究不同浓度斑蝥素和去甲斑蝥素对体外膀胱鳞状细胞癌细胞(BSCC)增殖的抑制作用,明确药物效果、最佳药物浓度及其抗肿瘤细胞增殖的机制。方法:使用不同浓度的斑蝥素和去甲斑蝥素与BSCC共同培养,MTT实验计算细胞生长抑制率,进而选出抑制肿瘤细胞的最佳药物及其最佳浓度。选用最佳浓度的最佳药物与肿瘤细胞共同培养,不同时间收集细胞,电子显微镜及激光共聚焦显微镜观察细胞形态。结果:在浓度<60μmol/L、作用时间≥24h情况下,斑蝥素对BSCC的抑制作用随着斑蝥素的浓度(剂量)增加和作用时间的延长而增加,具有明显的时间-效应和剂量-效应关系。斑蝥素具有较好的抑制BSCC增殖的作用,48h时药物半数抑制浓度IC50为60μmol/L。激光共聚焦扫描显微镜及电子显微镜观察证实浓度60μmol/L的斑蝥素具有明显促进BSCC细胞凋亡的作用。结论:斑蝥素具有较好的抑制BSCC增殖的作用,有望为BSCC化疗药物开拓新的思路。
Objective：Cantharidin（CA）is recently used as anti-tumor natural medicine in many cancers including bladder carcinoma,but the mechanisms are poorly understood.This study is aimed to explore the inhibit effects of CA and its derivative-norcantharidin（NCTD）on bladder squamous cell carcinoma（BSCC）in vitro.Method：Cultured BSCCs were treated with CA or NCTD at different concentrations for 12h,24h,36h,48h and 60h,then cell viability was measured by MTT assay.After treated by CA and NCTD at optimized concentrations,BSCC cellular morphological changes were also investigated by using Laser Scanning Confocal Microscope（LSCM）and Electron Microscope.Result：Both CA and NCTD were able to decrease BSCC viability in time dependent and dose dependent manners;the maximum effect dose was 60μmol/L.When cells were treated with CA or NCTD at 60μmol/L for 24hor 36h,apoptosis was induced in BSCC,more apoptotic bodies were founded in CA group.Conclusion：Our findings provide new details concerning the toxicity of CA and NCTD towards BSCC.Both CA and NCTD induced apoptosis in BSCC,CA showed more cytotoxic effect than to NCTD.CA will be helpful to medicine therapy of BSCC.This knowledge provides a basis for chemotherapeutic development of bladder carcinoma.
出处
《临床泌尿外科杂志》
2014年第7期620-624,共5页
Journal of Clinical Urology
基金
清华-周大福医学研究专项基金(编号202836019-04)
清华-裕元医学科学研究基金(编号20240000538)
关键词
斑蝥素
去甲斑蝥素
膀胱鳞状细胞癌
激光共聚焦显微镜
cantharidin
norcantharidin
bladder squamous carcinoma
laser scanning confocal microscope
