摘要
目的:探讨益气养血方对体外培养的小鼠胚胎癌细胞(ATDC5)软骨细胞Bcl-2/Bax蛋白及白细胞借宿(IL-1β)、肿瘤坏死因子(TNF-α)表达水平的影响,揭示其抗骨关节炎软骨细胞凋亡的机制。方法:将培养好的ATDC5小鼠软骨细胞分为空白组、空白+LPS组、双醋瑞因组、双醋瑞因+LPS组、益气养血方组、益气养血方+LPS组,干预24h后,观察益气养血方对ATDC5软骨细胞Bcl-2/Bax蛋白表达、软骨细胞凋亡及IL-1β、TNF-α的影响。结果:益气养血方能抑制ATDC5软骨细胞凋亡,下调细胞上清液炎性因子IL-1β、TNF-α产生水平(P<0.01,P<0.05),上调ATDC5软骨细胞Bcl-2蛋白表达,下调Bax蛋白表达水平。结论:调控Bcl-2/Bax平衡,抑制IL-1β、TNF-α释放,可能是益气养血方干预骨关节炎软骨细胞凋亡的机制之一。
Objective： To investigate the effects of Yiqi Yangxue Decoction on protein expression levels of ATDC5 chondrogenic Bcl-2/Bax, IL-1β and TNF-α cultured in vitro, and to reveal its anti-apoptotic mechanism on bone arthritis cartilage cells. Methods： The cultured ATDC5 mice cartilage cells were divided into six groups： the blank control group, blank ＋ LPS group, diacerein group, diacerein ＋ LPS group, Yiqi Yangxue group, Yiqi Yangxue ＋ LPS group. After 24 hours’ intervention, the protein expression of ATDC5 chondrocytes Bcl-2/Bax, the apoptosis of cartilage cells and the influence of IL-1βand TNF-α expression levels intervened by Yiqi Yangxue Decoction were observed. Results： Yiqi Yangxue Decoction can inhibit the apoptosis of ATDC5 chondrocytes, down-regulate the cytokine levels of IL-1β, TNF-α（P〈0.01, P〈0.05） and protein levels of Bax, up-regulate the protein expression of ATDC5 chondrocytes Bcl-2, mediate the ratio balance of Bcl-2/Bax. Conclusion： The regulation of Bcl-2/Bax balance and inhibition of the release of IL-1β and TNF-α may be one of the mechanisms of Yiqi Yangxue Decoction on the intervention of osteoarthritic cartilage cell apoptosis.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2016年第12期5202-5205,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81360542)~~
