摘要
目的构建多层次指标评价模型以优选红花通络方(HTP)最佳提取工艺路线。方法选取有效成分、杂质控制、行为学、组织病理学、免疫监测作为准则层,方中代表性成分羟基红花黄色素A、淫羊藿苷的提取率,出膏率,大鼠机械性刺激痛觉超敏反应、机械性刺激痛觉过敏反应的缩足百分率,背根神经节细胞偏心核比率,足底P物质量作为指标层,运用模糊层次分析法(FAHP)为各指标赋权,综合评价HTP各提取工艺路线。结果准则层指标权重(ω)由高到低为免疫监测(ω=0.245)、组织病理学(ω=0.23)、有效成分和行为学(ω=0.20)、杂质控制(ω=0.125)。最佳工艺路线为红花60℃温浸、淫羊藿等水回流提取、桂枝80%乙醇回流提取。结论以FAHP为赋权方法,有效成分指标结合药效学指标和杂质控制作为评价指标来优选提取工艺路线的方法,既能保留复方有效合理的本质,又能满足现代制剂研发的要求。
Objective To establish an optimization model for the extraction technology process of Honghua Tongluo Prescription（HTP） based on fuzzy analytic hierarchy process（FAHP）. Methods The evaluation indexes tree was established which included objective level, criterion level, and index level. The criterion level consisted of effective constituent, impurity control, behavioristics, histopathology, and immune response. The index level consisted the extraction efficiency of hydroxysafflor yellow A（HSYA） and icariin, the content of total substances, mechano-allodynia, and mechano-hyperalgesia, the ratios of decentered nucleolus in dorsal root ganglion, the content of SP in pelma skin with plantar P quality as the index layer. Weighting coefficient of all the indexes was decided by FAHP based calculation. HTP extraction process route was comprehensively evaluated. Results The importance degrees（ω） of each criterion from high to low were： immune response（ω = 0.245）, histopathology（ω = 0.23）, effective constituent, behavioristics（ω = 0.20）, and impurity control（ω = 0.125）. The optimized extraction technology process was that Carthami Flos was better extracted by warm maceration with water at 60 ℃, Epimedii Herba was circulated by reflux extraction with water. Cinnamomi Ramulus was better treated by reflux extraction with 80% alcohol. Conclusion An FAHP-based evaluation method with pharmacological indexes, effective constituent indexes, and impurity control as evaluation indexes, which could put formula Chinese materia medica principle and modern pharmaceutical engineering demands into consideration, is comprehensive and effective.
出处
《中草药》
CAS
CSCD
北大核心
2016年第17期3009-3015,共7页
Chinese Traditional and Herbal Drugs
基金
北京中医药大学自主选题项目(2015-JYB-XS076)
北京市科技计划"十病十药"研发项目(Z151100003815019)
关键词
红花通络方
模糊层次分析法
多指标评价
权重
提取工艺路线
羟基红花黄色素A
淫羊藿苷
Honghua Tongluo Prescription
fuzzy analytic hierarchy process
multi-criterion evaluation
weighting coefficient
extraction technology process
hydroxysafflor yellow A
icariin
