摘要
目的表现遗传学是当前肿瘤生物学研究的热点,本研究探讨食管鳞状细胞癌癌组织中组蛋白赖氨酸特异性脱甲基酶1(lysine specific demethylase 1,LSD1)和Zeste基因增强子(enhancer of zeste homolog 2,EZH2)的蛋白表达与术后预后的相关性。方法收集2008-05-07-2009-8-20在安阳市肿瘤医院胸外科行食管癌根治术的食管鳞癌患者85例。选取食管鳞癌组织85例,淋巴结转移癌组织30例,癌旁组织30例。免疫组织化学法检测食管鳞癌组织、淋巴结转移癌组织、癌旁组织中LSD1和EZH2蛋白表达。采用χ2检验分析LSD1和EZH2蛋白表达与临床病理特征的关系;Spearman法分析LSD1和EZH2两者的相关性;Kaplan-meier法和Logrank-test检验分析LSD1和EZH2蛋白表达与术后总生存期(overall survival,OS)和无进展生存期(progression free survival,PFS)的关系,Cox模型多因素预后分析。结果LSD1在食管鳞癌组织和淋巴结转移癌组织中的高表达率分别为64.7%(55/85)和70.0%(21/30);癌旁组织均为少量阳性表达(低表达),主要位于增殖较旺盛的鳞状上皮基底部;LSD1在食管鳞癌组织、淋巴结转移癌组织的表达均高于癌旁组织(P<0.001,P=0.001),食管鳞癌组织与淋巴结转移癌组织中的表达差异无统计学意义,P=0.598。EZH2在食管鳞癌组织、淋巴结转移癌组织和癌旁组织中的高表达率分别为62.3%(53/85)、76.7%(23/30)和20.0%(6/30);EZH2在食管鳞癌组织、淋巴结转移癌组织的表达均高于癌旁组织(P<0.001,P=0.001),食管鳞癌组织与淋巴结转移癌组织中的表达差异无统计学意义,P=0.155。Spearman相关分析显示,LSD1和EZH2蛋白表达存在正相关,r=0.239,P=0.028。LSD1蛋白表达水平与pT分期(P=0.007)和分化程度(P=0.021)相关。EZH2蛋白表达水平与pT分期(P=0.022)、pN分期(P=0.046)和分化程度(P=0.018)相关。单因素分析显示,LSD1高表达患者的mOS和mPFS均低于低表达患者,均P<0.001。EZH2高表达患者的mOS和mPFS均低于低表
OBJECTIVE Epigenetic is a hot research topic in tumor biology. This study is to investigate the relation- ship between protein expression of LSD1 and EZH2 and postoperative prognosis in esophageal aquamous cell carcinoma （ESCC）. METHODS The data from 85 patients with thoracic esophageal squamous cell carcinoma who underwent curative R0 esophagectomy from May 7,2008 to August 20,2009 in Anyang Cancer Hospital were retrospectively exam- ined. The LSD1 and EZH2 expression in ESCC tissues, lymph node metastasis carcinoma tissues and adjacent normal epi- thelia tissues were detected by immunohistochemical staining. Correlation of LSD1 and EZH2 expression and clinicopatho- logical factors were analyzed using Chi-squared test. Spearman method was used to analyze the correlation between LSD1 and EZH2. Survival curves were generated according to the Kaplan-meier method, and the statistical analysis was per- formed by Cox regression. RESULTS The high expression rates of LSD1 in esophageal squamous cell carcinoma tissues and lymph node metastasis carcinoma tissues were 64.7% （55/85）, 70.0% （21/30） respectively. The adjacent tissues were a few positive expression （low expression）, mainly in the squamous epithelial basal of relatively strong proliferation. The expression of LSD1 in ESCC tissues and lymph node metastasis carcinoma tissues were higher than in adjacent normal epithelia tissue （P〈0. 001 ;P= 0. 001）. Comparison of ESCC tissues and lymph node metastasis carcinoma tissues, the differences were not statistically significant （P=0. 598）. The high expression rates of EZH2 in esophageal squamous cell carcinoma tissues and lymph node metastasis carcinoma tissues were 62.3 % （53/85）, 76.7 % （23/30） and 20.0 % （6/30） respectively. The expression of EZH2 in ESCC tissues and lymph node metastasis carcinoma tissues were higher than that in adjacent normal epithelia tissue （P〈0. 001 ;P=0. 001） ; Comparison of ESCC tissues and lymph node metastasis carci- noma tissues, the differences
出处
《中华肿瘤防治杂志》
CAS
北大核心
2016年第14期936-943,951,共9页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金〔青年科学基金项目(81201945)〕
