摘要
目的:研究组蛋白赖氨酸特异性脱甲基酶1(lysine specific demethylase 1,LSD1)在食管鳞癌组织中的表达及其与临床病理因素的关系,并探讨其与预后的相关性。方法:收集2005-01-01-2006-12-31在福建省肿瘤医院胸外科接受食管癌三野根治术且术前未接受放疗或化疗的食管鳞癌患者135例。免疫组化检测135例食管鳞癌及其配对癌旁正常食管黏膜组织的LSD1表达水平。运用χ2检验分析肿瘤组织LSD1表达与临床病理因素的关系。运用Kaplan-Meier方法和Log-rank检验分析肿瘤组织LSD1表达与患者术后总生存时间的关系。采用Cox模型对食管癌患者预后相关因素进行多因素回归分析。结果:食管鳞癌组织LSD1强阳性表达率为53.3%,在正常食管黏膜组织中为7.6%,差异有统计学意义,χ2=9.016,P=0.002。LSD1高表达与性别(χ2=0.396,P=0.546)、年龄(χ2=2.530,P=0.123)、T分期(χ2=1.264,P=0.286)、淋巴结转移(χ2=1.136,P=0.343)、TNM分期(χ2=0.396,P=0.546)和分化(χ2=0.415,P=0.537)无显著相关性,而与生存时间是否超过5年(χ2=6.699,P=0.013)显著相关。Cox多因素回归分析显示,淋巴结转移(P=0.001)、肿瘤浸润深度(P=0.004)和LSD1表达水平(P=0.020)是食管鳞癌患者的独立预后因素。生存分析提示,LSD1强阳性组患者预后明显差于LSD1弱阳性组患者,P=0.008。亚组分析显示,在有淋巴结转移的患者中,肿瘤LSD1强阳性与患者预后相关,P=0.014;而在无淋巴结转移的患者中,LSD1强阳性与预后无显著相关性,P=0.373。结论:LSD1在食管鳞癌组织中表达上调,其强阳性表达与不良预后相关。
OBJECTIVE： To investigate the expression of LSD1 in esophageal squamous cell carcinoma （ESCC） and relationship of LSD1 and clinicopathological factors of ECSS patients. METHODS： The data from 135 patients with tho racic esophageal squamous cell carcinoma who underwent curative R0 esophagectomy with three-field lymph node dissec tion from January lst,2005 to December 31st, 2006 in Fujian Cancer Hospital were retrospectively examined. The LSD1 expression in tumors and adjacent normal epithelia from 135 ESCC patients was detected by immunohistochemical stai ning. Correlations of LSD1 expression and clinicopathological factors were analyzed using C-squared test. Survival curves were generated according to the Kaplan-Meier method,and the statistical analysis was performed by Log-rank test. Multi- variate analysis were also performed to assess the element which affect the survival of patients by Cox regression. RE- SULTS： The expression of LSD1 protein was significantly up-regulated in tumors with 53.3% strong positive staining, compared with paired adjacent normal epithelia with 7.6% strong positive staining （P=0. 002）. No correlation was found between expression of LSD1 in tumor cells and clinicopathologic factors such as gender （X2 = 0. 396, P = 0. 546）, age （？（2 =2. 530,P=0. 123） ,tumor invasion （c2 =1. 264,P=0. 286） ,lymph node metastasis （3（2 = 1. 136,P=0. 343）,TNM stage （X2 = 0. 396, P = 0. 546） and tumor differentiation （3（2 = 0. 415, P = 0. 537）. Strong positive staining of LSD1 in tumor cell was correlated with 5-year survival rate （x2 = 6. 699, P 0. 013）. Cox-proportional multivariate analysis showed that expression of LSD1 was a significant prognostic factor in overall survival（P= 0. 020）. The survival curves calculated by Kaplan-Meier method further showed that the patients with strong LSD1 expression had a shorter survival than patients
出处
《中华肿瘤防治杂志》
CAS
北大核心
2014年第4期280-283,288,共5页
Chinese Journal of Cancer Prevention and Treatment
