摘要
目的检测EZH2、增殖细胞核抗原(PCNA)、P16蛋白在胃癌及癌旁正常组织中的表达情况,分析EZH2与患者临床病理特征及预后的关系。方法收集62例胃癌手术患者的肿瘤及52例癌旁正常胃粘膜组织石蜡标本进行免疫组织化学染色,并收集患者的临床病理特征及随访资料,同时检测PCNA、P16蛋白的表达,分析这3种蛋白质之间的关系;分析EZH2与胃癌患者临床病理特征及预后的关系。结果胃癌组织中EZH2、PCNA表达均较癌旁组织明显增强(P<0.05),P16蛋白在胃癌组织中表达明显低于癌旁组织阳性率(P<0.05);胃癌组织中EZH2与PCNA表达有正相关关系(r=0.3846,P=0.002),EZH2与P16蛋白表达存在负相关关系(r=-0.2756,P<0.05);PCNA与P16表达无明显相关关系(P>0.05)。EZH2表达与胃癌肿瘤浸润深度、分化程度、TNM分期、淋巴结转移有关(P<0.05);预后分析显示,EZH2强表达患者的生存期比弱表达者明显缩短(P<0.05)。结论胃癌组织中EZH2蛋白表达明显增高,与胃癌部分临床病理特征有关,EZH2蛋白还可以作为预后指标;EZH2蛋白在胃癌中的作用可能与其调节肿瘤细胞增殖能力有关。
Objective To investigate the significance of expression of EZH 2, proliferating cell nuclear antigen （PCNA）,P16 in gastric cancer tissues and its correlation with clinicopathological characteristics and prognosis of patients . Methods The expression levels of EZH2,PCNA,P16 were tested by immunohistochemistry in 62 cases of gastric cancer tissues and 52 cases of tumor-adjacent tissues , and patients ’ clinicopathological characteristics and follow-up data were collected.The correlation among the three kinds of proteins was analyzed , and the relationship between EZH 2 and clinicopathological characteristics as well as prognosis of patients was analyzed .Results The expression levels of EZH2 （69.35%） and PCNA （83.87%） in gastric cancer tissues were significantly higher than those （21.15%,38.46%） in tumor-adjacent tissues （ P ＆lt;0.05）,however,the expression levels of P16 （33.87%） in gastric cancer tissues were significantly lower than those in tumor-adjacent tissues （73.08%, P ＆lt;0.05）.The expression levels of EZH2 were positively correlated with those of PCNA in gastric cancer tissues ,however,the expression levels of EZH2 were negatively correlated to those of P16 （ P ＆lt;0.05）,moreover,there was no obvious relationship between the expression of PCNA and that of P 16 （ P ＆gt;0.05）.The overexpression of EZH2 was related to the depth of tumor infiltration,differentiation degree,clinical TNM stage, lymph node metastasis （ P ＆lt;0.05）.Prognosis analysis showed that that the survival duration in patients with overexpression of EZH2 was obviously shorter than that of patients with weak expression of EZH 2 （ P ＆lt;0.05 ）.Conclusion The overexpression of EZH2 appears in gastric cancer tissues , which is related to clinicopathological characteristics of gastric cancer,moreover,EZH2 protein can be served as an index of prognosis evaluation ,and its effect in gastric cancer may be correlated to regulating proliferation ability of tumor cells .
出处
《河北医药》
CAS
2014年第4期492-494,共3页
Hebei Medical Journal
