摘要
该实验通过建立大鼠下腔静脉血栓模型和小鼠急性肺血栓模型,考察鬼箭锦鸡儿醇提物(TE)的抗血栓作用。通过考察TE的2个主要部分TE-1和TE-2对小鼠急性肺血栓的作用,以及比较TE、TE-1和TE-2对出血时间和凝血时间的影响,探索其抗血栓活性部位。通过对照品对照结合液相色谱-质谱分析对提取物中所含6种主要成分及其他成分的种类或类别进行鉴定。结果表明,TE各剂量组均能够显著降低大鼠下腔静脉血栓质量(P<0.01),中、高剂量时能够提高对急性肺血栓模型小鼠的保护率(P<0.05)。TE-2在保护小鼠免于偏瘫或死亡、延长出血时间和凝血时间方面均比TE-1显示出较强活性。TE中主要含有异黄酮、紫檀素及茋类化合物,TE-2中以异黄酮和紫檀素类成分为主,TE-1中以茋类为主,提示这3类成分可能均为鬼箭锦鸡儿抗血栓的活性成分。
The antithrombotic effect of Caragana jubata（ Pall.） Poir. ethanolic extract（ TE） was evaluated by inferior vena cava thrombosis in rats and acute pulmonary thrombosis in mice. To search for the bioactive fractions of TE,comparison on acute pulmonary thrombosis was made between the two main fractions of TE（ TE-1 and TE-2）. Besides,pharmacological effects of TE,TE-1 and TE-2 on bleeding time and clotting time were also studied. Reference substances combined with UPLC / DAD-q-TOF-MS were applied to identify the main six compounds and other chemical constituents of the TE. The results showed that TE could significantly reduce the rat thrombosis weight in all doses（ P〈0. 01） and improve the protective rate to mice in medium and high doses（ P〈0. 05）. TE-2showed a stronger effect on protecting the mice from paralysis or death and prolonging the bleeding time and clotting time than TE-1.Chemical constituents in TE mainly include isoflavones,pterocarpans and stilbenoids. Constituents in TE-2 were mainly isoflavones and pterocarpans,while those in TE-1 were mainly stilbenoids,which could be inferred that all of these three kinds of constituents may be responsible for the antithrombotic effects of Caragana jubata.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第13期2473-2480,共8页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81473425)
