摘要
为研究6-苄氨基腺嘌呤(6-BA)对蝴蝶兰花梗休眠芽萌发的影响,以及花梗腋芽不同木质化程度对于休眠芽萌发率的影响,以3个大花型红花蝴蝶兰品种‘大辣椒’(Phalaenopsis‘Big Chili’)、‘0436’(P.‘Red Swan’)以及‘阿里山姑娘’(P.‘Alishan Guniang’)的含休眠芽的花梗节段为试材,对外植体进行组织培养,统计外植体休眠芽萌发率以及生长情况。研究表明:(1)花梗腋芽半木质化时休眠芽萌发率最高,可达100%。(2)在所试用的培养基中,MS+6-BA 5.0 mg/L培养基最适宜品种‘0436’以及‘阿里山姑娘’的休眠芽萌发,MS+6-BA 4.0 mg/L培养基最适宜品种‘大辣椒’的休眠芽萌发。试验成功获得了3个品种最适宜花梗休眠芽萌发的外植体木质化程度,以及花梗休眠芽萌发的最佳培养基种类,为不同品种的大花型红花蝴蝶兰组培快繁生产体系的建立提供理论依据。
The paper aims to study the influence of 6-benzylaminopurine（6-BA） on germination of sleepingbuds on stalks of moth orchid（Phalaenopsis） varieties, and the influence of sleeping buds with different degreesof lignification on the success rate of the bud germination. In this experiment, three varieties of‘Big Chili＇,‘Red Swan＇and‘Alishan Guniang＇were cultured in vitro, to count up the success rate of germination and thegrowth situation of the stalk sleeping buds. The results indicated that：（1） when the buds were in halflignification, stalk had the hightest success rate of germination, which was up to 100%;（2） in all culturemediums, MS＋6-BA 5.0 mg/L was the most suitable one for the buds germination of‘Red Swan＇and‘AlishanGuniang＇, and MS＋6-BA 4.0 mg/L was the most suitable one for the buds germination of‘Big Chili＇. Theexperiment successfully obtained the most appropriate lignification degrees for the sleeping buds germinationon stalks of the three varieties, and the most appropriate culture mediums of the sleeping buds germination,therefore, it could provide certain theoretical basis for establishing a production system of tissue culture andrapid reproduction of different varieties of moth orchid with large red flowers.
出处
《中国农学通报》
2016年第16期87-92,共6页
Chinese Agricultural Science Bulletin
基金
山东省良种产业化项目"玉簪
蝴蝶兰等种质资源保存
创新利用及繁育技术体系研究"
