摘要
以蝴蝶兰的叶片、花梗腋芽、花梗节间、茎尖、根尖为外植体,通过对蝴蝶兰组织培养的不同类型的基本培养基及各种激素配比进行组合试验,筛选出蝴蝶兰组织培养的最适外植体及其培养基配方。试验结果表明:花梗腋芽是蝴蝶兰组培的最佳外植体,其理想培养基配方为:1/2MS+2 mg/L 6-BA+0.2 mg/L NAA+0.3%Ac+20 g/L蔗糖+8 g/L琼脂,原球茎诱导率可达72.9%。
The leaves, pedicel axillary bud, pedicel lintemode, stem tip and root tip of butterfly orchid were used as explants, and the best explant and culture medium were screened by testing different culture medium with hormone combination. The result showed that the best explant was pedicel axil/ary bud,and the best culture medium was 1/2 MS medium supplemented with 6 -BA 2 mg/L ＋ NAA 0.2 mg/L ＋ Ac 0.3% ＋ cane sugar 20 g/L ＋ agar 8 g/L. The protocorm induction rates of pedicel axillary bud was 72. 9%.
出处
《江西农业学报》
CAS
2008年第9期51-53,共3页
Acta Agriculturae Jiangxi
基金
辽宁工程技术大学青年基金项目(2005B)
关键词
蝴蝶兰
组织培养
原球茎
外植体
Butterfly orchid
Tissue culture
Protocorm
Explant
