摘要
目的:探讨榆干离褶伞(Lyophyllum ulmarium,L.u)溶栓酶对H_2O_2诱导人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)损伤的保护作用。方法:用H_2O_2诱导HUVEC氧化损伤,采用四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法观察细胞存活率;用流式细胞仪检测细胞线粒体跨膜电位(ΔΨ_m)和总活性氧(reactive oxygen species,ROS)水平;Western blotting法检测Caspase-8、Caspase-9、Caspase-3等蛋白的表达水平。结果:L.u溶栓酶可显著提高HUVEC的存活率,明显抑制H_2O_2诱导的细胞内ROS生成和线粒体跨膜电位的下降,并能降低Caspase-8、Caspase-9和Caspase-3的表达水平。结论:L.u溶栓酶对氧化应激损伤的HUVEC有保护作用,其机制可能与抑制细胞凋亡有关。
Objective： To observe the protective effect of fibrinolytic enzyme from Lyophyllum ulmarium（L.u） on H_2O_2-induced oxidative damage of human umbilical vein endothelial cells（HUVECs）. Methods： H_2O_2 was used to induce the oxidative injury model of HUVECs. The survival rate of HUVECs was determined by MTT assay. The mitochondrial transmembrane potential（ΔΨ_m） and reactive oxygen species（ROS） of HUVECs were detected by flow cytometry. The expression of Caspase-8, Caspase-9 and Caspase-3 proteins was detected by Western blotting. Results： L.u could increase the survival rate of endothelial cells, inhibit intracellular ROS production and mitochondrial transmembrane potential reduction in HUVECs, and down-regulate the expression of Caspase-8, Caspase-9 and Caspase-3 proteins. Conclusion： The fibrinolytic enzyme from Lyophyllum ulmarium has protective effect on vascular endothelial cells due to the inhibition of apoptosis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第1期171-175,共5页
Food Science
基金
国家自然科学基金面上项目(30760150
81360088)
关键词
榆干离褶伞
溶栓酶
人脐静脉内皮细胞
线粒体跨膜电位
活性氧
凋亡
Lyophyllum ulmarium
fibrinolytic enzyme
human umbilical vein endothelial cell
mitochondrial transmembrane potential
reactive oxygen species
apoptosis
