摘要
目的确定影响开心散药物质量的关键因素,优选出开心散的最佳药物制备物形式,为创制开心散优质抗痴呆药物奠定基础。方法采用所建立的HPLC分析法测定开心散制备物有效指标性成分的含量。基于制备物主要有效指标性成分的含量、含量相对比值、相对制备量以及出膏率和抗痴呆药效对开心散药物制备物的质量进行系统表征,并将质量表征结果进行关联分析。结果基于药物体系所建立的开心散有效指标性成分的含量测定方法操作简便、准确、重复性好,可用于开心散药物制备物质量表征研究。开心散3种药物制备物形式均表现出抗痴呆作用,其作用大小为:富集物>稀醇提取物>水提取物。通过开心散药物制备物药学-药效质量表征关联分析可知,开心散富集物中有效指标性成分的含量最高、比例独特、出膏率低、药物用量最小,改善学习记忆作用最强,为开心散抗痴呆药物最佳制备物形式。结论基于开心散的基本组成及其制备得率,探寻药物成分的最适含量及其相对比值,是提升药物药效的关键因素。
Objective To determine the key factors affecting the quality of Kaixin San Heart-opening powder and the best preparation forms of Kaixin San,laying the groundwork of anti-dementia drug devel-opment from Kaixin San.Methods The content of effective index components of medicinal-preparation of Kaixin San was detected by using HPLC.The quality of medicinal preparation from Kaixin San were characterized based on the content,relative quantity,relative ratio of content,yield rate and anti-dementia effect,and the characterized results were given correlation analysis.Results The method of the determi-nation of effective index components from Kaixin San is easy to handle,accurate and highly reproducible. The effect of anti-dementia of three medicinal preparations from Kaixin San was listed in the following de-scending order of enrichment,ethanol extract,and water extract.The content of effective index compo-nents is higher with unique proportion,lower extraction rate,lower dosage and higher antibacterial effect, which is the best form in medicinal-preparation application of Kaixin San.Conclusion The key to the improvment of efficacy is to explore the fittest content and relative ratio of medicinal components based on the basic composition of Kaixin San and yield rate.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2015年第8期539-545,F0003,共8页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.81102776)
十二五国家科技支撑计划项目(No.2012BA129B06)
北京中医药大学创新团队项目(No.2011-CXTD-12)
关键词
药物体系
开心散药物制备物
质量表征
关联分析
drug system
medicinal-preparation of Kaixin San
quality characteristics
correlation analysis
