摘要
目的建立基于药物体系的藁本质量表征及其关联分析方法,为中药质量有效评价提供新模式。方法采用HPLC同时测定7批藁本饮片中阿魏酸、洋川芎内酯A、洋川芎内酯H、洋川芎内酯Ⅰ、藁本内酯等5种有效指标性成分的含量,分析基于有效指标性成分含量的质量表征;以洋川芎内酯Ⅰ含量为参比,对饮片进行有效指标性成分含量相对比值质量表征,并以批次4(已确定药效的饮片)为基准,作有效指标性成分含量相对比值的比值差,其绝对值之和表示为非关联系数δ,分析得关联度排序(δ/关联度)。结果基于有效指标性成分含量的质量表征:以阿魏酸含量排序为批次7>批次6>批次4>批次3>批次1>批次5>批次2,含量相差最大约2.2倍;以洋川芎内酯Ⅰ含量排序为批次7>批次4>批次3>批次6>批次1>批次5>批次2,含量相差最大约4.9倍;以苯酞类含量和排序为批次7>批次3>批次6>批次4>批次1>批次2>批次5,含量相差最大约3.6倍;批次7、3、4、6有效指标性成分总体含量较高。基于关键有效指标性成分含量相对比值的质量表征:批次4(0/100%)>批次7(1.21/79.8%)>批次3(1.36/77.3%)>批次6(1.83/69.5%)>批次5(2.18/63.7%)>批次1(2.28/62.0%)>批次2(9.14),即批次7、3的质量表征与批次4关联度较高。综合基于有效指标性成分含量及其相对比值的质量表征,以批次4为基准,可得批次7、3质量较优。结论基于有效指标性成分含量及其相对比值进行质量表征,并以具有确切药效的药材饮片为基准,进行质量表征关联度分析,以评价其质量整体关联性与应用有效性,这是中药质量评价提供一种新模式。
Objective To establish the quality representation of Gaoben （Rhizoma Ligustici ） and correlation analysis method based on drug system, and provide a new mode for quality evaluation of Chinese medicinal. Methods HPLC method was used for simultaneous determination of 5 effective in- dex components in 7 batches of Gaoben decocting pieces, including ferulic acid, senkyunolide A, senkyunolide H, senkyunolide I, and ligustilide, and then a correlation analysis was carried out;The quality representation based on the content relative ratio of key effective index components showed as follows： taking the content of senkyunolide I as reference, the quality representations of decocting pieces were dete^nined based on the content relative ratio of effective index components, and using batch 4 （decocting pieces with identified therapeutic effect） as baseline for getting the difference between these relative ratio of effective index components, and its sum of absolute value represented as non-correlation coefficient. Results The quality representation based on the content of effective index components showed as follows ： by the content of ferulic acid, the sorting was batch 7 〉 batch 6 〉 batch 4 〉 batch 3 〉 batch 1 〉 batch 5 〉 batch 2 and the maximum content difference was about 2.2 times ; by the content of senkyunolide I, the sorting was batch 7 〉 batch 4 〉 batch 3 〉 batch 6 〉 batch 1 〉 batch 5 〉 batch 2 and the maximum content difference was about 4.9 times; by the total content of phthalides, the sorting was batch 7 〉 batch 3 〉 batch 6 〉 batch 4 〉 batch 1 〉 batch 2 〉 batch 5 and the maximum content difference was about 3.6 times. The total content of effective index components was higher in batch 7, batch 3, batch 4 and batch 6. The sorting of the correlation degree （ ~/correlation degree） was analyzed as batch 4 （0/100%） 〉 batch 7 （ 1.21/79.8% ） 〉 batch 3 （ 1.36/77.3% ） 〉 batch 6 （ 1.83/69.5% ） 〉 batch 5 （2.18/63.7%） 〉 batch 1 （2.28/62.0%�
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2014年第7期481-485,I0001,共6页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家十二五科技支撑计划项目(No.2012BAI29B06)
北京中医药大学创新团队资助项目(No.2011-CXTD-12)
北京中医药大学重点学科开放课题(2013-2DXKKF-23)
关键词
藁本
质量评价
关联分析
阿魏酸
洋川芎内酯A
洋川芎内酯H
洋川芎内酯I
藁本
内酯
Gaoben （ Rhizoma Ligustici ）
quality evaluation
correlation analysis
ferulic acid
senkyunolide A
senkyunolide H
senkyunolide I
ligustilide
