摘要
目的:研究木槿叶的化学成分和抑制α-葡萄糖苷酶活性。方法:通过大孔树脂柱、硅胶柱色谱、Sephadex LH-20色谱等技术分离化合物,通过理化数据和1H-NMR、13C-NMR鉴定化合物结构,并以96微孔板检测活性。结果:从木槿叶乙醇提取物中鉴定了15个化合物,分别为:β-谷甾醇(1)、β-胡萝卜苷(2)、β-香树脂醇(3)、齐墩果酸(4)、豆甾-4-烯-3-酮(5)、木栓酮(6)、syriacusin A(7)、山柰酚(8)、异牡荆素(9)、牡荆素(10)、芹菜素(11)、芹菜素-7-O-β-D-葡萄糖苷(12)、木犀草素-7-O-β-D-葡萄糖苷(13)、牡荆素-7-O-β-D-葡萄糖苷(14)、芦丁(15)。结论:以上化合物均为首次从木槿叶中分离得到。以阿卡波糖为阳性对照,α-葡萄糖苷酶抑制活性成分筛选结果显示,化合物7和9对α-葡萄糖苷酶活性抑制作用较强,其IC50分别为39.03±0.38、32.12±0.62 mg/L,抑制率分别达到94.95%、97.15%。
Objective： To study the chemical constituents from Hibiscus syriacus leaves and their α-glucosidase inhibitory activities. Methods： Column chromatography including macroporous resins,silica gel and Sephadex LH-20 were used for the isolation and purification of all compounds. Spectroscopic methods including physical and chemical properties,1H-NMR and13C-NMR were used for the identification of structures. Their α-glucosidase inhibitory activities were detected by a 96-well microplate. Results： 15 compounds were isolated and identified as β-sitosterol（ 1）,β-daucosterol（ 2）,β-amyrin（ 3）,oleanolic acid（ 4）,stigmast-4-en-3-one（ 5）,friedelin（ 6）,syriacusin A（ 7）,kaempferol（ 8）,isovitexin（ 9）,vitexin（ 10）,apigenin（ 11）,apigenin-7-O-β-D-glucopyranoside（ 12）,luteolin-7-O-β-D-glucopyranoside（ 13）,vitexin-7-O-β-D-glucopyranoside（ 14） and rutin（ 15）. Conclusion： All the compounds are isolated from the leaves of Hibiscus syriacus for the first time. Taking acarbose as positive control,the α-glucosidase inhibitory activities of 15 compounds were evaluated. Compounds 7 and 9 have shown strong α-glucosidase inhibitory activities with IC50 of 39. 03 ± 0. 38 and 32. 12 ± 0. 62 mg / L,inhibition ratio of 94. 95% and 97. 15%,respectively.
出处
《中药材》
CAS
CSCD
北大核心
2015年第5期975-979,共5页
Journal of Chinese Medicinal Materials
基金
安徽新华学院校级质量工程项目(2014jgkcx14)
国家大学生创新创业训练项目(201312216029)
关键词
木槿叶
化学成分
Α-葡萄糖苷酶抑制剂
Hibiscus syriacus L.
Chemical constituents
α-Glucosidase inhibitory activities
