摘要
目的采用超滤亲和-液质联用(Ultra-filtration affinity-liquid chromatography-mass spectrometry,UF-LC-MS)和分子对接技术筛选毛菊苣Cichorium glandulosum根中α-葡萄糖苷酶抑制剂。方法通过超滤亲和-液质联用技术筛选并鉴定毛菊苣根中的α-葡萄糖苷酶抑制剂,发现有4个组分对α-葡萄糖苷酶的活性具有抑制作用,进一步采用分子对接技术对这4个化合物进行验证。结果发现2个化合物对α-葡萄糖苷酶具有较高的抑制作用,其中黄芩苷、山莴苣苦素在0.1～2.0mg/mL呈良好的量效关系。结论为利用UF-LC-MS和分子对接技术筛选天然产物中α-葡萄糖苷酶抑制剂提供了参考,也为后续研究毛菊苣降糖功效提供了基础。
Objective To screen α-glucosidase inhibitors from root extract of Cichorium glundulosum(CGR) by UF-LC-MS and molecular docking.Methods The inhibitory activity of CGR extract was evaluated by establishing enzyme inhibitor model,Meanwhile,four active compounds from CGR extract were screened by UF-LC-MS and identified as α-glucosidase inhibitors,which were verified by using Autodock Software.Results The results showed that two compounds that combined with α-glucosidase well were screened out,including baicalin and lactupicrin,which showed significant α-glucosidase inhibitory activity in the concentration range of 0.1-2 mg/mL in a dose-dependent manner.Conclusion Our work provide theoretical basis for screening natural and high affinity enzyme inhibitors from CGR by using UF-LC-MS and molecular docking and further study of the hypoglycemic effect of C.glundulosum.
作者
陈海君
马尚智
江敏
王秋平
藏洁
秦惠玉
陈文
韩博
CHEN Hai-jun;MA Shang-zhi;JIANG Min;WANG Qiu-ping;ZANG Jie;QIN Hui-yu;CHEN Wen;HAN Bo(Key Laboratory of Xinjiang Phytomedicine Resource and Utilization,Ministry of Education,School of Pharmacy,Shihezi University,Shihezi 832002,China;School of Chemistry and Chemical Engineerir/g,Shihezi University,Shihezi 832002,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第2期344-351,共8页
Chinese Traditional and Herbal Drugs
关键词
毛菊苣
超滤亲和-液质联用
分子对接
Α-葡萄糖苷酶抑制剂
黄芩苷
山莴苣苦素
Cichorium glundulosum Boiss.et Huet
ultrafiltration affinity-liquid chromatography-mass spectrometry
molecular docking
a-glucosidase inhibitors
baicalin
lactupicrin
