摘要
目的制作"泻剂结肠"大鼠模型,并观察其胃、小肠、结肠组织中c-kit mRNA的表达变化。方法健康Wistar雄性大鼠20只,随机分为实验组与对照组,每组各10只。实验组给予大黄酸粉悬液灌胃制作"泻剂结肠"模型,对照组予生理盐水灌胃。造模期间每日观察大鼠粪便形状。造模结束、灌胃停止1周后,以活性炭悬液灌胃,计算活性炭末推进率,据此判断造模是否成功。采用real-time PCR法检测胃窦、小肠、结肠组织中的c-kit mRNA。结果实验组大鼠大便性状较对照组变硬。实验组与对照组活性炭末推进率分别为39.24%±4.28%、61.84%±3.05%,两组相比,P<0.05。实验组"泻剂结肠"模型制作成功。实验组大鼠胃窦、小肠、结肠组织中c-kit mRNA相对表达量分别为0.61±0.08、0.37±0.02、0.32±0.03,对照组分别为0.67±0.03、0.90±0.05、1.01±0.09,两组小肠及结肠组织中c-kit mRNA相对表达量相比,P均<0.05。结论采用大黄酸粉悬液灌胃法成功制作了"泻剂结肠"大鼠模型,其小肠、结肠组织中c-kit mRNA表达下调,可能与"泻剂结肠"的形成有关。
Objective To establish the rat model of cathartic colon and to observe the expression changes of c-kit mR-NA in the stomach, small intestine and colon tissues.Methods Twenty healthy male Wistar rats were randomly divided into two groups：the control group and constipated group, ten in each group.Rhein suspension was administered to induce cathartic colon in the constipated group.The control group was intragastrically treated with normal saline.During the mod-eling, we observed the shape of rat stool daily.At the end of modeling or one week after the end of intragastric administra-tion, rats were treated with activated carbon suspension, and we calculated the activated carbon transit rate to see if the modeling was successful or not.Then, Then c-kit mRNA expression in the gastric antrum, small intestine and colon tissues were detected by real-time PCR.Results The stool in the constipated group was significantly harden as compared with that of the control group.Carbon black transit rates in the constipated group and control group were 39.24%±4.28%and 61.84%±3.05%, respectively;and significant difference was found between the two groups （P〈0.05）.The rat model of cathartic colon was successful in the constipated group.The expression of c-kit mRNA in the gastric antrum, small intes-tine and colon tissues of the constipated group was respectively 0.61 ±0.08, 0.37 ±0.02 and 0.32 ±0.03, and they were 0.67 ±0.03, 0.90 ±0.05 and 1.01 ±0.09 in the control group.Significant difference was found in the c-kit mRNA ex-pression of the small intestine and colon tissues between the two groups （all P〈0.05）.Conclusion The rat model of ca-thartic colon could be successfully established with rhein, and the c-kit mRNA expression in the small intestinal and colon tissues is up-regulated which may be related with the pathogenesis of cathartic colon.
出处
《山东医药》
CAS
北大核心
2015年第27期4-6,共3页
Shandong Medical Journal
基金
北京市自然科学基金资助项目(7122180)
关键词
便秘
慢传输型便秘
泻剂结肠
CAJAL间质细胞
酪氨酸激酶生长因子受体
动物试验模型
constipation
slow transit constipation
cathartic colon
interstitial cells of Cajal
tyrosine kinase growth factor receptors
animal experimental model
