摘要
[目的]建立豚鼠结肠黑变病动物模型。[方法]15只豚鼠随机分为5组:大黄1组:大黄2 g/(kg.d)灌服30 d;大黄2组:大黄2 g/(kg.d)灌服60 d;大黄3组:大黄4 g/(kg.d)灌服30 d;大黄4组:大黄4 g/(kg.d)灌服60 d及正常组。处死动物,观察结肠黑变情况,行组织大体评分,取结肠组织行黑色素染色、黑色素腿色实验,并对盲肠及近段结肠未染色切片行图像分析。[结果]各大黄组豚鼠结肠均有不同程度黑变,以盲肠及近段结肠为著。病理改变类似人类。结肠黑色素染色阴性,黑色素褪色染色阳性。图像分析显示,各大黄组盲肠及近段结肠色素颗粒灰度显著低于正常组,且与大黄剂量(盲肠r=-0.861,结肠r=-0.772,P<0.01)及时间(盲肠r=-0.749,结肠r=-0.785,P<0.01)呈负相关;色素颗粒面积比显著高于正常组,且与大黄剂量(盲肠r=0.810,结肠r=0.791,P<0.01)及时间(盲肠r=0.663,结肠r=0.549,P<0.01)呈正相关。[结论]用大黄可致豚鼠实验性结肠黑变病,黑变程度与大黄剂量及时间相关。该模型制作简单、重复性好,可用于多种实验研究。
[Objective]To establish a model of melanosis coli with rhubarb in guinea pig. [Methods] Fifteen guinea pigs were randomly divided into 5 groups. Rhubarb 1 group received a daily intragastric administration of rhubarb 2 g/kg for 30 days, rhubarb 2 group was given rhubarb 2 g/kg for 60 days. Rhubarb 3 group 4 g/kg for 30 days and rhubarb 4 group 4 g/kg for 60 days. Normal group drank water freely. The animals were then killed, color of colon was ob- served and score of degree of nigrities was evaluated. Melanin stain and color fading stain of melanin were performed in colon sections. Image analysis was performed in unstained sections of cecum and proximal colon. [Results] Macroscopically, the mucosa of the cecum and proximal colon was brown in color in different degree in the animals receiving rhubarb. Pathological change closely resembled human melanosis coli. Stainning for melanin was negative. Color fading stain of melanin was positive. Image analysis showed the grey scale of pigments of cecum and proximal colon in every rhubarb group was lower than normal group significantly and had negative correlation with dose （cecum r=-0. 861, colon r=-0. 772, P〈0. 01） and time （cecum r=-0. 749, colon r=-0. 785, P〈0. 01） of using rhubarb. Area% of pigments of cecum and proximal colon in every rhubarb group was higher than normal group significantly and had positive correlation with dose （cecum r=-0. 810, colon r=-0. 791, P〈0. 01） and time （cecum r=0. 663, colon r= 0. 549, P〈0. 01） of using rhubarb. [Conclusion] Rhubarb can induce melanosis coli in guinea pig. The degree of nigrities correlated with dose and time of using rhubarb. Model building is simple and can be reproduced. This model can be applied in various studies of melanosis coli.
出处
《中国中西医结合消化杂志》
CAS
2006年第5期308-311,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
关键词
疾病模型
动物
结肠黑变病
色素
大黄
disease models, animal
melanosis coli
pigment
rhubarb
