摘要
目的:研究多巴胺D2受体(dopamine D2 receptor,Drd2)在慢传输型便秘(slow transit constipation,STC)大鼠消化系组织表达情况,探讨引发STC大鼠便秘发病的可能机制.方法:选取24只健康Wistar大鼠随机分成2组,实验组和对照组.实验组每天予以复方苯乙哌啶混悬液(8 mg/kg)灌胃制造慢传输便秘大鼠模型,对照组给予相等剂量的生理盐水灌胃.每5 d记录1次大便粒数、大便干质量及大鼠体质量.饲养90 d后停药1 wk,判断造模成功与否.大鼠解剖后分别选取胃窦、小肠及结肠组织,采用实时荧光聚合酶链反应法(real-time polymerase chain reaction,RT-PCR)检测组织中Drd2的表达情况.结果:通过测定比较实验组与对照组大鼠的日均粪便粒数、日均粪便质量及首粒黑便排出时间,差异均有统计学意义,判断STC大鼠造模成功.RT-PCR能特异性扩增Drd2,Drd2在S T C大鼠胃、小肠组织中的表达与对照组的表达差异无统计学意义(3.97±1.21,P>0.05;3.12±1.14,P>0.05),而在结肠组织中的表达明显高于对照组(1.93±0.78,P<0.05),差异有统计学意义.结论:STC大鼠Drd2在胃、小肠组织表达与正常大鼠的表达差异无统计学意义,在结肠组织表达显著上调,提示Drd2可能参与STC大鼠的发病机制.
AIM： To investigate the expression of dopamine D2 receptor （Drd2） in the gastrointestinal mucosa of rats with slow transit constipation （STC） to explore the possible pathogenesis of STC. METHODS： Twenty-four healthy Wistar rats were randomly into two groups： an experiment group and a control group. Rats of the experiment group were daily administered diphenoxylate （8 mg/kg） for 90 d to induce STC, while the control rats were fed normal saline. The number and weight of fecal granules and the body weight of rats were recorded every 5 d. After successful induction of STC, the rats were killed to take tissues of the gastric antrum, small intestine, and colon. Real-time polymerase chain reaction （RT- PCR） was used to detect the expression of Drd2 in the above tissues. RESULTS： The daily number and mean weight of fecal granules and the time to discharge of the first black granule differed significantly between the two groups, suggesting that STC was successfully induced. Drd2 was specifically amplified by RT-PCR. Compared with the control group, the expression of Drd2 in the stomach and small intestine was not significantly different in the experiment group （P 〉 0.05 for both）. However, the expression of Drd2 in colon tissues of STC rats was significantly higher than that in control rats （1.93 ± 0.78, P 〈 0.05）. CONCLUSION： The expression of Drd2 in the stomach and small intestine does not change significantly in STC rats, but Drd2 expression is up-regulated in colon tissues of STC rats, which may contribute to the pathogenesis of STC.
出处
《世界华人消化杂志》
CAS
2015年第1期93-98,共6页
World Chinese Journal of Digestology
