摘要
目的:应用大黄酸及大黄建立大鼠泻剂结肠模型,二者进行比较,寻找一种简单、稳定的建立泻剂结肠动物模型的药物.方法:健康3-4周龄Wistar大鼠33只,均分为对照组、大黄组、大黄酸.对照组给予生理盐水灌胃;大黄组给予大黄粉悬液灌胃;大黄酸组给予大黄酸粉悬液灌胃.造模完成后采用活性炭悬液推进法测定大鼠肠道传输功能.结果:大黄组、大黄酸组造模时间分别为136d和114 d;大黄组及大黄酸组大鼠活性炭末推进长度及推进率较对照组明显缩短.大黄酸组造模过程中大鼠稀便稳定,且给药剂量及造模周期同参考文献报道一致.结论:大黄及大黄酸均可成功建立大鼠泻剂结肠模型,但作为单体大黄酸药效更稳定,浓度易于控制,且造模时间相对较短,故作为造模药物,效果优于大黄.
AIM： To find a simple and stable drug for the in- duction of cathartic colon by comparing two rat models which were established with rhein and rhubarb, respectively. METHODS： Thirty-three healthy 3-4-week- old Wistar rats were divided into three groups, namely, a control group, a rhubarb group and a rhein group. Normal saline was given by in- tragastric administration in the control group. Rhubarb suspension was given in the rhubarb group, and rhein suspension was given in the rhein group. The intestinal transit function was assessed using the activated carbon suspension transit method.RESULTS： Modeling time was 136 and 114 d for the rhubarb group and rhein group, re- spectively. Activated carbon transit length in the rhubarb and rhein group was significantly shorter than that in the control group. The symptom of loose stools in the rhein group was stable in the modeling process, and the dose and modeling cycle were consistent with the reference literature. CONCLUSION： A rat model of cathartic colon could be successfully established with both rhu- barb and rhein. As a monomer, the efficacy of rhein is more stable than rhubarb. Concentration of rhein is easy to control and it is associated with a relatively short period of time in estab- lishing the animal model. Rhein is better than rhubarb in establishing the rat model of cathartic colon.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第9期1262-1265,共4页
World Chinese Journal of Digestology
基金
北京市自然科学基金资助项目
No.7122180~~
关键词
泻剂结肠
动物模型
大黄
大黄酸
单体
Cathartic colon
Animal model
Rhu-barb
Rhein
Monomer
