摘要
便秘动物模型对便秘机制研究和通便药物研发有至关重要的作用,其中造模方法的选择尤为关键。通过查阅文献,整理归纳慢传输型便秘鼠类动物模型常见造模方法,将从药物(复方地芬诺酯、洛哌丁胺、吗啡、硫糖铝、粪菌移植、大黄、大黄酸、番泻叶提取物)、非药物(限水、低纤维膳食)及中医常见证型造模(气滞、湿热、实热、津亏、气虚、阳虚、阴虚、血虚)3方面进行介绍。结果发现,药物造模仍以复方地芬诺酯、洛哌丁胺多见且方法相对成熟,但存在造模剂量、时长、稳定性不明的问题;大黄、大黄酸诱导的泻剂结肠动物模型造模周期较长,但符合临床治疗现状,值得进一步研究;非药物造模自限性风险高,适用于生活习惯对STC影响的研究;中医证型造模以“物理刺激+药物干预”的复合模式为主,但量效指标较主观,无法客观评价证型造模效果。
The animal model of constipation plays a crucial role in the study of constipation mechanisms and the development of laxatives,with the selection of modeling methods being particularly crucial.By reviewing literature and summarizing common modeling methods for slow transit constipation in rodent models,this article introduced three aspects:drug[compound diphenoxy-late,loperamide,morphine,sucralfate,fecal microbiota transplantation,Dahuang(rhubarb),rhein,and senna extract],non drug(water restriction,low fiber diet),and common TCM syndrome modeling(Qi stagnation,damp-heat,excess heat,body fluid defi-ciency,Qi deficiency,Yang deficiency,Yin deficiency and blood deficiency).The results showed that compound diphenoxylate and loperamide were still commonly used in drug modeling and the methods were relatively mature,but there were issues with un-clear modeling dosage,duration and stability.The animal model of laxative colon induced by Dahuang(rhubarb)and rhein has a long modeling cycle,but it is in line with the current clinical treatment status and deserves further research.Non pharmacological modeling has a high risk of self limitation and is suitable for studying the impact of lifestyle habits on slow transit constipation.The traditional Chinese medicine syndrome modeling mainly adopts a composite model of“physical stimulation+drug interven-tion"',but the dose-response indicators are subjective and cannot objectively evaluate the effectiveness of syndrome modeling.
作者
卢艺丹
方萍萍
姚柱豪
骆云丰
LU Yidan;FANG Pingping;YAO Zhuhao;LUO Yunfeng(The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350003,Fujian,China)
出处
《实用中医内科杂志》
2024年第3期109-113,共5页
Journal of Practical Traditional Chinese Internal Medicine
基金
福建省自然科学基金项目(2021J01878)
福建省卫生健康委员会中医处科技计划项目(2021zylc44)。
关键词
慢传输型便秘
动物模型
中医证候
综述
slow transit constipation
animal model
TCM syndromes
review
