摘要
目的探讨大段感染骨煮沸后异位活化的可行性。方法将160只健康6个月龄中国白兔随机分为实验组、对照组各80只。实验组：首先制备兔胫骨感染模型，然后截取2．0cm长感染胫骨，经煮沸灭菌后，异位于对侧大腿股直肌与股内侧肌间隙之隐动脉处，1．0克氏针固定于股骨卜对照组：存同一部位截取相同长度的无菌胫骨段经生理盐水浸泡后，余步骤同实验组。两组分别于术后各时间节点处死10只兔，通过大体标本观察及免疫组织化学染色法检测移植骨骨形态发生蛋白—2（BMP-2）和I型胶原蛋白生成量的灰度值，分析判断两组骨活化进程。结果自体正常骨忡化肟逐渐被结缔组织包裹，其表面出现大小不同的小凹，且逐渐增多，要快于煮沸骨相应进程。对照组BMP-2和I型胶原蛋向的灰度值在术后10周达到峰值，分别为147．04±9．52、148．87±6．52，与实验组术后16周相应数值（145．08±6．59、147．25±5．49）比较，差异均无统计学意义（P〉0.05）。结论兔自体正常骨异位于血供丰富的组织间隙内经过10周可转变为活化骨，要快于煮沸竹，而煮沸骨经过16周也完成活化过程。证实兔大段感染骨煮沸后异位活化的可行性。
Objective To investigate the possibility of activation about long intectious bone after boiling by ectopic transplantation. Methods 160 healthy Chinese rabbits （6 months of age） were random- ly divided into experimental group and control group with 80 rabbits per group. Experimental group： the rabbit tibia infection model was established first, and then 2.0 cm long infectious tibia was cut out and boiled which was ectopically implanted into the contralateral thigh rectus femoris and vastus medialis muscle gap of the rabbit around the saphenous artery by fastened to the femur by 1.0 Kirschner - wire. Control group： cut out the same part and length of sterile tibia treated with normal saline soak, another steps with the experimental group. The 10 rabbits from two groups respectively at all time nodes after surgery were put to death and bone activation process of two groups were analyzed by general observation of samples and im- munohistochemical staining method to detect grey value of bone morphogenetic protein-2 （BMP- 2） and collagen I expression of the bone grafts. Results The autologous normal bone by ectopic transplantation was gradually wrapped in the fibrous connective tissue and its surface appeared small concaves of different sizes with gradually increasing, and these were faster than boiling bone process accordingly. 10 weeks post- operatively, the gray values of BMP -2 and collagen I of the control group peaked and were respectively （147.04±9.52, 148.87 ± 6. 52）, which were compared with the corresponding numerical （145.08 ±6. 59, 147.25 ±5.49） of the experimental group 16 weeks postoperatively, with no statistically significant diference （ P 〉 0. 05 ）. Conclusion The rabbit autologous normal bone located in the organization gap of the rich blood supply needed 10 weeks into activated bone and was faster than boiling bone, and boiling bone also completed the activated process at the postoperative 16 weeks. The experiment confirmed the pos- sibility of activation about long inf
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第8期1940-1942,共3页
Chinese Journal of Experimental Surgery
基金
河南省科技攻关计划重点资助项目（102102310002）
关键词
感染骨
煮沸骨
异位
骨活性
骨形态发生蛋白-2
I型胶原
Infectious bone
Boiling bone
Ectopic, bone activity
Bone morphogenetic protein - 2
Collagen I
