摘要
目的 制备新型的缓释万古霉素脱蛋白松质骨支架并研究其性能.方法 利用静电纺丝技术将载万古霉素的纳米纤维膜贴附于牛脱蛋白松质骨支架表面.通过扫描电镜观察处理前后骨支架的表面特征、高效液相色谱法检测载药支架的万古霉素释放速率、抑菌实验观察载药支架的体外抗菌效率,支架与兔间充质干细胞共培养观察对细胞增殖的影响.结果 载万古霉素纳米纤维膜紧密贴附于脱蛋白松质骨支架表面,纤维直径约100 ～1 200 nm,表面孔径大小约1～5 μm.体外万古霉素释放实验结果显示洗脱液中第1天的药物浓度最高,随后逐渐缓慢降低,但维持在最小抑菌浓度以上的时间超过4周.体外抑菌实验结果证实了活性万古霉素的存在,并且有效抑菌圈可以维持至4周.兔间充质干细胞共培养实验结果显示载药、非载药支架和空白组在1、3、6、9、14d时,相对细胞生存系数先降低,在第6天达到最低约93.8％,以后观察时间均出现不同程度的升高,各组间的细胞增殖速度差异无统计学意义（P＞0.05）.结论 新型缓释万古霉素脱蛋白松质骨支架可以缓慢释放活性万古霉素,有效抑菌时间长,且细胞相容性好,是修复感染性骨缺损的理想支架材料.
Objective This study describes the preparation and characteristics of a novel biodegradable vancomycin-eluting nanofiber-loaded bovine deproteinized bone scaffold.Methods The solution of poly （D,L）-lactide-co-glycolide （PLGA） and vancomycin in 1,1,1,3,3,3-hexafluoro-2-propanol was prepared and then electrospun into nanofibers onto the surface of bovine deproteinized bone scaffold.The surface characteristic of the novel scaffold was scanned by scanning electron microscope.The in vitro release rate of vancomycin from the nanofiber-enveloped scaffolds was detected by an elution method.The in vitro antibacterial activity and cytocompatibility was also investigated.Results Vancomycin-eluting nanofiber membrane has a diameter of about 100-1 200 nm and a pore size of about 1-5 μm,closely attached on the surface of deproteinized bone scaffold.Vancomycin-eluting scaffolds released a sustained high concentrations of vancomycin in vitro （well above the minimum inhibitory concentration） for more than 4 weeks.The bacterial inhibition zone lasts for about 4 weeks.When the scaffold materials were co-cultured with rabbit mesenchymal stem cells,relative cell survival factor declined slowly to the minimum of about 93.8％ on the 6th day,and then increased slowly,which had no significant difference statistically among three groups.Conclusion The new vancomycin-eluting nanofiber-loaded deproteinized bone scaffold can achieve sustained release of antibiotics,good cytocompatibility,and thus may be an ideal repair material for the prevention and treatment of infected bone defect.
作者
高建廷
黄国锋
刘国浚
任磊
陈长青
丁真奇
Gao Jianting Huang Guofeng Liu Guojun Ren Lei Chen Changqing Ding Zhenqi(Department of Orthopedic, Affiliated Dongnan Hospital of Xiamen University, the 175th Hospital of PLA, Zhangzhou 363099, China Department of Biomaterials , College of Materials, Xiamen University, Xiamen 361005, Chin)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第11期2451-2454,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金（NSFC81401783）
南京军区医学科技创新课题（112022）
军分部医学科技青年基金（18FBQN2014006）
