摘要
采用超滤分级、离子层析和分子筛凝胶层析方法,系统纯化龙须菜粗多糖,分离到多糖GCp F1–1B。经HPLC鉴定,GCp F1–1B为均一多糖,分子量为9.3×104 Da。紫外扫描显示其不含蛋白和核酸。高效阴离子色谱(HPAEC)分析发现,GCp F1–1B由半乳糖、果糖和葡萄糖醛酸组成,比例为14.28∶3.85∶1。生物活性研究显示,GCp F1–1B可以刺激小鼠脾淋巴细胞增殖作用和激活小鼠骨髓来源巨噬细胞(BMMs)的作用,表明其具有良好的免疫调节活性。
GCpFI-IB was isolated form crude polysaccharide of Gracilaria lemaneiformis by ultra- filtration, ion chromatography and gel filtration chromatography. HPLC showed that GCpFI-IB was homogeneous and its molecular weight was 9.3- 104 Da. UV scanning indicated that GCpFI-IB contained no protein and nucleic acid. The monosaccharide compositions of GCpF 1-1B were made up of galactose, fructose and glucuronic acid by high performance anion exchange chromatography （ HPAEC ）, and the ratio was 14.28 ： 3.85 ： 1. Bioactivity assay demonstrated that GCpFI-IB could stimulate the mouse spleen lymphocytes （ MSLs ） to proliferate and activate bone marrow macrophages （ BMMs ） in a dose-dependent manner, indicating a good immunological activity on GCpF 1-1B.
出处
《粮食与油脂》
北大核心
2015年第7期65-68,共4页
Cereals & Oils
基金
国家自然科学基金(31301596)
关键词
龙须菜
多糖
纯化
免疫活性
Gracilaria lemaneiformis
polysaccharides
purification
immunocompetence
