摘要
采用维生素C和过氧化氢体系诱导产生的自由基降解龙须菜多糖,并对龙须菜多糖及其降解产物的抗氧化活性进行分析。结果表明,在维生素C和过氧化氢反应体系中,龙须菜多糖的降解反应在2 h内基本完成,降解后得到的4种产物分子量分别为21 492,12 864,5 417和3 043 u,降解反应未导致龙须菜多糖去硫酸化,但3,6-内醚-半乳糖含量略有降低;在清除超氧阴离子自由基、DPPH自由基和还原Fe3+方面,龙须菜多糖的抗氧化活性随分子量的降低而增强,而在清除羟基自由基方面,抗氧化活性却随多糖分子量的降低而减弱。
To discuss the effect of polysaccharide molecular weight on the antioxidation, Gracilaria lemaneiformis polysaccharide was degraded by free radical from ascorbate and hydrogen peroxide in combination, then the antioxidative activity of G. lemaneiformis polysaccharide and its degraded products was determined. The results showed that G. lemaneiformis polysaccharide was degraded basically by the reaction system of ascorbate and hydrogen peroxide in two hours, and four kinds of degraded polysaccharide were achieved with molecular size of 21 492,12 864,5 417 and 3 043 u, respectively. In the degradation, the sulfate contents were not changed but 3,6-anhydro-galactose contents of degraded products decreased a little. Moreover, the higher the molecular weight of G. lemaneiformis polysaccharide, the weaker the power of reducing Fe^3＋, scavenging superoxide anion radical （O2^-.） and 1, 1-diphenyl-2-picrylhydrazyl （DPPH） radical. However, the inhibitory activity againest hydroxyl radical （ .OH） decreased with the degradation.
出处
《水产学报》
CAS
CSCD
北大核心
2009年第2期342-347,共6页
Journal of Fisheries of China
基金
国家“八六三”高技术研究发展计划(2003AA625030)
宁波市科技局科研攻关项目(2004C100034)
关键词
龙须菜多糖
降解
抗氧化活性
分子量
Gracilaria lemaneiforrnis polysaccharide
degradation
antioxidation
molecular weight
