摘要
目的：探究黄芩苷对大鼠全脑缺血/再灌注损伤后动物行为学、内源性神经干细胞再生数量以及COX-2（环氧化酶-2）蛋白表达量的影响,分析其可能的作用机制。方法：84只体质量250-300 g Wistar大鼠按随机数字表法分为3个实验组（n=28）：假手术组（Sham）,全脑缺血/再灌注后黄芩苷胃灌注处理组（Baicalin）,全脑缺血/再灌注后生理盐水胃灌注处理组（Saline-water）。参照Pulsinelli法建立全脑缺血/再灌注损伤大鼠模型,通过Bederson神经症状分级评分、被动回避实验、免疫组织化学检测、蛋白免疫印迹实验分别检测黄芩苷对模型大鼠神经功能损伤、学习记忆能力、内源性神经干细胞增殖数量以及COX-2蛋白表达变化的影响。结果：与假手术组（Sham）比较,Baicalin组与Saline-water组大鼠神经功能障碍症状都更加明显且差异显著;大鼠进入暗室的潜伏期均缩短,错误次数均明显增多;海马脑区5-溴脱氧尿嘧啶核苷（Brd U）阳性细胞数均显著增加;脑组织环氧合酶-2（COX-2）蛋白的表达显著增加。与Saline-water组比较,Baicalin组大鼠经黄芩苷胃灌注干预,神经功能损伤显著改善,潜伏期显著延长,错误次数显著减少;Baicalin组海马脑区Brdu阳性细胞数显著多于Saline-water组,Baicalin组COX-2蛋白的表达水平较Salinewater组明显降低。结论：黄芩苷对大鼠全脑缺血/再灌注损伤具有显著保护作用,其机制可能是通过降低COX-2基因表达、抑制损伤伴随炎症反应,从而改善内源神经干细胞小生境（niche）,促进内源性神经干细胞的增殖或存活率。
To investigate the effects of baicalin on animal behavior,proliferation of endogenous neural stem cells and COX-2 gene expression in hippocampus of rats after global cerebral ischemia / reperfusion injury and the probable mechanism involved. Methods： Eighty-four Wistar rats weighting 250-300 g were randomized into three experimental groups（ n = 28） ： Sham operation group（ Sham）; Intragastric administration of Baicalin after global cerebral ischemia/reperfusion surgery group（ Baicalin）; Intragastric administration of Saline-water after global cerebral ischemia / reperfusion surgery group（ Saline-water）. Model of global cerebral ischemia / reperfusion was established according to Pulsinelli method.Bederson neurological grading score system,passive avoidance test,immunohistochemistry analysis,western blot analysis were used to detect the effects of baicalin on rat neurological function,learning and memory capacity,endogenous neural stem cell proliferation as well as the protein expression of COX-2 after surgery. Results： Compared with the Sham group,the improvement of neurological dysfunction of rats in Baicalin group and Saline-water group were more pronounced,and the difference was significant; escape latency were shorter,number of errors were significantly increased; the number of Brdu positive cells in the hippocampus were significantly increased; expression of COX-2 protein was significantly increased in rat brain tissue. Compared with Saline-water group, intragastric administration Brdu in Baicalin group significantly improved the neurological damage; escape latency was significantly increased and also significantly reduced the number of errors; The number Brdu positive cells in Baicalin group was significantly higher than that in Saline-water group; expression of COX-2 protein in Baicalin group was significantly lower. Conclusions： Baicalin has a significant protective effect on global cerebral ischemia / reperfusion injury in rats,which may be related with reducing the COX-2 gene expr
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2015年第6期660-664,共5页
JOURNAL OF BASIC CHINESE MEDICINE
基金
黑龙江省教育厅科研项目(12531825)
