摘要
本试验旨在研究递增30%注射脂多糖（ LPS）诱导仔猪慢性免疫应激的效果及对仔猪生长性能、营养物质表观消化率的影响。选取16头断奶仔猪,随机分为2组（对照组、LPS组）,单笼饲养。 LPS组隔天静脉注射LPS,初始注射剂量80μg/kg BW,随后逐次递增30%,对照组注射相应剂量的生理盐水,试验期15 d。研究发现：仔猪注射LPS第1、5天血浆白细胞介素-1β和皮质醇含量极显著高于对照组（P〈0.01）,第9天血浆皮质醇有高于对照组的趋势（P=0.10）,整个试验期内血浆结合珠蛋白含量显著高于对照组（ P〈0.05）。与对照组相比,注射LPS仔猪在试验的3个阶段（1～4 d、5～8 d和9～15 d）平均日增重分别降低了40.00%（ P〈0.01）、29.52%（P〈0.05）和19.30%（P〈0.05）,平均日采食量分别降低了25.09%（P=0.01）、23.15%（ P〈0.05）和19.47%（ P〈0.05）,增重耗料比与对照组无显著差异（ P〉0.05）。与对照组相比,注射LPS仔猪饲粮干物质表观消化率下降了5.43%（P〈0.05）,能量表观消化率有降低的趋势（P=0.06）,粗脂肪和粗蛋白质表观消化率略有降低（P〉0.10）。试验结果表明,递增30%注射LPS成功诱导仔猪慢性免疫应激,降低了仔猪平均日采食量和营养物质表观消化率,抑制了仔猪生长。
This experiment was conducted to study the effects of gradually increasing 30% of lipopolysaccha-ride （ LPS） by injecting on the chronic immunological stress and on growth performance and nutrient apparent digestibility of piglets. Sixteen weaned piglets were randomly allotted into 2 groups （ control and LPS groups） , and were individually housed in metabolic cages. Saline （ control） or LPS were injected at 48 h intervals with an initial dosage of 80 μg/kg BW, increased by 30% at each following injections. The experiment lasted for 15 d. The results showed that the contents of interleukin-1β and cortisol in plasma of the LPS group were sig-nificantly higher than those of control group on days 1 and 5 （ P〈0.01） , and there was a trend that the content of cortisol in plasma of LPS group was higher than that of control group （ P=0.10） . Moreover, the content of haptoglobin in plasma was significantly higher than that of control group during the whole experimental period （ P〈0.05） . Compared with the control group, during three phases of this experiment （ from 1 to 4 days, 5 to 8 days and 9 to 15 days） , the average daily gain of LPS group was decreased by 40.00% （ P〈0.01） , 29.52%（ P〈0.05） and 19.30% （ P〈0.05） , respectively. For the average daily feed intake was decreased by 25.09%（ P=0.01） , 23.15% （ P〈0.05） and 19.47% （ P〈0.05） , respectively. At last, no significant difference was found in gain to feed ratio （ P〉0.05） . Compared with control group, the apparent digestibility of dry matter in LPS group was decreased by 5.43% （ P〈0.05） , and gross energy apparent digestibility had a decreasing trend （ P=0.06） , apparent digestibility of crude protein and ether extract were decreased slightly （ P〉0.10） . In con-clusion, increasing of 30% injection of LPS successfully induces the chronic immunological stress, and reduces feed intake and nutrient apparent digestibility of piglet, as well as inhibites the growth of piglets.
出处
《动物营养学报》
CAS
CSCD
北大核心
2015年第6期1868-1874,共7页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家“973”重点基础研究发展计划(2012CB124700)
中国农业科学院创新工程(ASTIP-IAS07)资助
关键词
仔猪
免疫应激
生长性能
表观消化率
LPS
LPS
piglets
immunological stress
growth performance
apparent digestibility
