摘要
为探讨发酵中药对免疫应激肉鸡血液指标的影响。选用7日龄健康AA肉鸡200只,随机分为5组:对照组(基础日粮)、LPS应激组(基础日粮+LPS)、1%未发酵中药组(基础日粮+LPS+1%未发酵中药)、0.5%发酵中药组(基础日粮+LPS+0.5%发酵中药)和1%发酵中药组(基础日粮+LPS+1%发酵中药)。除对照组外,其他各组分别在15、17、19、21、23、25、27日龄时腹腔注射LPS 100、200、200、250、250、400、400μg/(kg·bw),对照组注射等量的生理盐水。最后一次注射后24 h采集血液,测定血液常规和血清生化指标。结果显示:(1)与对照组相比,LPS应激组肉鸡的血液白细胞数目显著升高(P<0.05),血清中总蛋白、球蛋白、尿素氮和总胆固醇含量明显上升(P<0.05);谷丙转氨酶(ALT)、谷草转氨酶(AST)、肌酸激酶(CK)等血清酶活性显著升高(P<0.05)。3个中药组总蛋白和白蛋白含量均显著高于对照组(P<0.05)。(2)与LPS应激组相比,2个发酵中药组白细胞数目和AST血清酶活性显著下降(P<0.05),1%发酵中药组ALT和0.5%发酵中药组CK的血清酶活性显著下降。表明LPS应激对肉鸡血液常规和血清生化指标均有一定影响,添加中药能够显著降低肉鸡炎症反应,调控蛋白质和脂肪代谢,减轻免疫应激损伤,发酵中药优于未发酵中药。
To investigate the effects of fermented Chinese medicine on growth performance and immune function of broilers in immune stress,200 healthy 7-day-old AA broilers were randomly divided into control group(basal diet),LPS stress group(basal diet+LPS),1% unfermented Chinese medicine group(basal diet+LPS+1% unfermented Chinese medicine),0.5% fermented Chinese medicine group(basal diet+LPS+0.5% fermented Chinese medicine),and 1% fermented Chinese medicine group(basal diet+LPS+1% fermented Chinese medicine),respectively.Except the control group,the remaining groups were injected intraperitoneally with LPS at the ages of 15,17,19,21,23,25,27 days and the LPS concentrations were 100,200,200,250,250,400μg/(kg·bw)and 400μg/(kg·bw)respectively,while the control group was injected with the same amount of normal saline.Blood samples were collected 24 hours after the last injection and blood routine and serum biochemical indexes were measured.The results showed as follows:(1)Compared with the control group,the number of leukocytes,total protein,globulin,urea nitrogen and total cholesterol in serum were significantly decreased in LPS stress group(P<0.05).Glutamic-pyruvic transaminase(ALT),glutamic oxaloacetylase(AST)and creatine kinase(CK)in serum were increased(P<0.05);(2)Compared with LPS stress group,the number of WBC and the activity of AST serum enzyme in the two fermented Chinese medicine groups were significantly decreased(P<0.05).The activity of CK serum enzyme in the 1% fermentation group and the 0.5% fermentation group was significantly decreased(P<0.05).The results indicate that the LPS stress has certain effects on blood routine and serum biochemical indexes of broilers.Adding Chinese medicine could significantly reduce inflammation,regulate protein and fat metabolism and immune stress injury.Fermented Chinese medicine was superior to unfermented Chinese medicine.
作者
靳双星
张桂枝
王晶
任晓丽
张爱国
JIN Shuang-xing;ZHANG Gui-zhi;WANG Jing;REN Xiao-li;ZHANG Ai-guo(Henan University of Animal Husbandry and Economy,College of Veterinary Medicine,Zhengzhou 450046,China;Zhengzhou Poultry Diseases Diagnosis and Control Engineering Technology Research Center,Zhengzhou 450046,China;Henan University of Animal Husbandry and Economy,College of Animal Science and Technology Medicine,Zhengzhou 450046,China)
出处
《中国兽医杂志》
CAS
北大核心
2020年第10期59-63,共5页
Chinese Journal of Veterinary Medicine
基金
河南省重大科技专项计划项目(161100510200)
河南省重点研发与推广专项(192102110183)。
关键词
发酵中药
免疫应激
肉鸡
血液指标
fermented Chinese medicine
immune stress
broiler
blood index
