摘要
目的研究银杏叶提取物(GBE)对大鼠脾脏和胸腺免疫功能的影响。方法给SD大鼠灌胃给予(40、120、360)mg/(kg·d)GBE,同时设置对照组。28 d后,水合氯醛麻醉处死大鼠,测量胸腺和脾脏的质量指数;MTT法检测伴刀豆球蛋白A(Con A)诱导的大鼠脾淋巴细胞增殖转化;中性红实验测定大鼠腹腔巨噬细胞吞噬功能;扫描电镜观察大鼠脾脏和胸腺的超微结构变化。结果不同剂量的GBE均可提高胸腺和脾脏的器官质量指数,不同剂量组之间无显著性差异;不同剂量的GBE均可增强大鼠腹腔内巨噬细胞的吞噬作用及各级淋巴细胞的增殖能力,且呈现一定的剂量依赖性。电镜观察发现不同剂量的GBE处理组,大鼠脾脏和胸腺中成熟淋巴细胞数均较对照组增多。结论银杏叶提取物可增强大鼠胸腺和脾脏的免疫功能。
Objective To study the effect of Ginkgo biloba extract （GBE） on the immune function of spleen and thymus in SD rats. Methods Forty SD rats were randomly divided into four groups （10 rats each group）. Three experimental groups were given GBE daily by gavage in doses of 40, 120, 360 mg/（ kg · d）, respectively. Animals in the control group were fed the same amount of PBS. After 28 days, the rats were sacrificed by chloral hydrate anesthesia. The spleen and thymus were harvested to determine the organ index first. M＇IF assay was used to detect the concanavalin A （ ConA）-induced splenic lymphocyte proliferation and transformation. Neutral red assay was performed to measure the rat peritoneal macrophage phegocytosis. The ultrastructural changes of spleen and thymus were observed under scanning electron microscope. Results Administration of GBE in the rats increased the mass indexes of rat thymus and spleen, dose-dependently elevated the lymphocyte proliferative responses and enhanced the peritoneal macrophage phagocytosis. In experimental groups, the numbers of mature spleen and thymus lymphocytes were significantly raised in comparison with the control rats. Conclusion GBE plays a regulatory role in immune function of the rat by increasing the mass of immune organs, increasing the number of mature T lymphocytes as well as their proliferative responses, and enhancing the phagocytic capacity of peritoneal macrophages.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2015年第6期792-795,799,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(31160221)
