摘要
目的 观察大鼠胸腺内注射自体脑细胞诱导免疫耐受对于手术脑损伤的治疗效果.方法 24只SD雄性大鼠随机分为3组,每组8只,A组：手术脑损伤治疗组,B组：生理盐水对照组,C组：假手术组;术后1、3、7、14、21 d行改良大鼠神经功能缺陷评分（MNSS）、头颅核磁（MRI）检测脑水肿体积;酶联免疫吸附试验（ELISA）法检测外周血转化生长因子-β1（TGF-β1）、γ-干扰素（IFN-γ）浓度,流式细胞仪检测外周血CD4 ＋/CD8＋T细胞比值.结果 与B组比较,A组大鼠神经功能恢复明显增快、脑水肿时限明显缩短（P＜0.05）,其脑水肿体积（mm3）分别为[（39.41 ±3.68、80.91 ±7.50、92.25±7.06、74.53±7.05、47.80±10.00）、（42.21±4.11、87.07±4.70、108.56±10.29、97.58±12.75、65.96±11.85）];A组血清TGF-β1浓度（ng/L）在术后1、3、7d呈升高趋势,且均数高于B组（P＜0.05）,浓度（ng/L）分别为[（751.96±50.24、780.99±50.23、853.20±14.81）、（704.55±36.89、724.54±18.72、804.13 ±50.07）];A组IFN-γ浓度在术后7、14、21 d呈下降趋势且均数低于B组（P＜0.05）,浓度（ng/L）分别为[（132.60±19.89、129.89 ±21.12、106.34±23.91）、（164.43 ±32.52、179.98 ±40.21、157.46 ±50.68）];A组CD4 ＋/CD8＋T比值在术后7、14 d呈下降趋势,在14d最低（P＜0.05）,比值为1.43±0.20;与C组比较,B组TGF-β1浓度在3、7、14 d呈升高趋势且均值较高（P＜0.05）,浓度（ng/L）分别为[（724.54±18.72、804.13±50.07、808.33 ±62.12）、（646.66±94.53、652.62±98.80、619.39 ±89.20）],IFN-γ浓度在1、3、7、14d呈升高趋势且均值较高（P＜0.05）,其浓度（ng/L）分别为（113.38±15.42、133.56±26.10、164.43±32.52、179.98 ±40.21）、（83.08±12.70、87.76±14.10、103.27±22.19、97.29± 13.98）];B组CD4 ^＋/CD8^＋T比值在3、7、1d持续升高,比值分别为1.68 ±0.31、2.00±0.30、2.20±0.27.结论 胸腺内注射自体脑细胞诱导免疫耐受可减轻手�
Objective To explore the curative effect after surgical brain injury （SBI） by develop an immune system that is tolerant to auto-cerebral cells through thymus tolerance.Methods 24 male SD rats were randomly divided into three groups,8 in each group：A group：SBI models and treatment group,B normal saline control group,C Sham group,functional outcome was evaluated using modified neurological severity score （MNSS）,cerebral edema was detected by magnetic resonance imaging （MRI）,transforming growth factor-β1 （TGF-β1）,interferon gamma （IFN-γ） were detected by enzyme linked-immuno-sorbent assay （ELISA）,CD4 ＋/CD8 ＋ T ratio in peripheral blood detected by flow cytometry （FCM） at 1,3,7,14,21 d after SBI.Results A group MRI showed less cerebral edema,neurological evaluation confirmed functional outcomes were significantly improved compared with B group,cerebral edema volume respectively （mm3） were [（39.41±3.68,80.91 ±7.50,92.25 ±7.06,74.53 ±7.05,47.80 ± 10.00）,（42.21 ±4.11,87.07 ±4.70,108.56 ± 10.29,97.58 ± 12.75,65.96 ± 11.85）] ; in A group,levels of TGF-β1 were obviously increased at 1,3,7 d and the mean were higher than B group （P 〈 0.05）,concentration respectively （ng/L） were [（751.96 ± 50.24,780.99 ± 50.23,853.20 ± 14.81）,（704.55 ± 36.89,724.54 ± 18.72,804.13 ± 50.07）] ; Concentration of IFN-γ significantly declined at 7 14 21 d,and the mean were lower than B group （P 〈0.05）,concentration（ng/L） respectively were [（132.60 ± 19.89,129.89 ±21.12,106.34 ±23.91）,（164.43 ±32.52,179.98 ±40.21,157.46 ± 50.68）] ; CD4 ＋/CD8 ＋ T ratio in group A were significantly decreased at 7,14 d,and the mean was lowest at 14 d time point （P 〈 0.05）,the ratio were （1.43 ± 0.20） ; B group showed a worsen functional outcomes compared with C Group,levels of TGF-β1 in group B were obviously increased at 3,7,14 d and the means were higher than C group （P 〈 0.05）,concentration （ng/L） respectively were [（724.54±18
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第3期538-541,共4页
Chinese Journal of Experimental Surgery
基金
天津市自然科学基金资助项目（13JCYBJC21100）
天津市科技支撑计划资助项目（13ZCZDSY01600）
