摘要
目的探讨口服免疫耐受对脑损伤的治疗作用。方法58只兔随机分为对照组、脑脊液引流组、脑脊液引流＋口服组和免疫佐剂组。收集第1、3、7、14、21天血清，以ELISA法检测IL-1、IFN-γ、IL-10、TGF-β和ABAb浓度，并进行对比。结果脑脊液引流组Ⅱ、-1、IFN-γ、ABAb较对照组降低，IL-10较对照组升高；脑脊液引流＋口服组IL-1、IFN-γ、ABAb较其他组明显降低，而IL-10、TGF-β明显升高；免疫佐剂组介于对照组和脑脊液引流＋口服组之间。结论脑脊液引流对颅脑创伤有治疗作用；口服免疫耐受对颅脑创伤有治疗作用；完全弗氏佐剂部分抑制了口服免疫耐受。
Objective To explore the cerebral protective effects of oral tolerance therapy in rabbit- TBI - models established by lateral fluid percussion machine. Method 58 rabbits were divided into four groups randomly（ 18 in control group, 15 in CSF drained group, 10 in CSF drained ＋ feed group and 15 in CSF drained ＋ feed ＋ immunologic adjuvant group）. Rabbit＇s CSF were drained and injected into cistern in control group; were drained only in CSF drained group;were drained and feed by mouth in drained ＋ feed group;were drained and injected with 50 μg complete Freud＇s adjuvant under the skin of posterior foot and rabbits in drained ＋ feed ＋ immunologic adjuvant group. 1 ml CSF was drained everyday until a week and phlebotomized and tested with ELISA to observe the changes of IL- 1 ,IFN -γ,IL- 1O,TGF - β and ABAb at the time points of 1 d,3 d ,7 d, 14 d ,21 d in each groups. Results Levels of IL - 1 ,IFN - γ and ABAb in CSF drained group were lower and levels of IL - 10 were higher than that in control group;levels of IL - 1, IFN -7 ,ABAb in CSF drained ＋ feed group were lower and levels of IL - 10 ,TGF - β were higher than that in any other groups significantly; levels of IL - 1, IFN - γ, ABAb in CSF drained ＋ feed ＋ inmmnologic adjuvant were lower than that in eontrol group,while they were higher than that in CSF drained ＋ feed group; levels of IL - 10 and TGF - β in CSF drained ＋ feed ＋ immunologic adjuvant group were higher than that in control group,while they were lower than that in CSF drained ＋ feed group. Statistic differences among groups were significant（ P 〈 O. 05 ）. The mortality rate and disability rate of CSF drained ＋ feed group were the lowest,while the cure rate was the highest among four groups. The control group came to be the worst outcome. And the other two groups were in the middle. Conclusions CSF drainage can help to cure traumatic brain injury. CSF drainage and feed by mouth can result in oral tolerance and lighten the injury, so oral tolerance is a
出处
《中华神经外科杂志》
CSCD
北大核心
2011年第5期624-627,共4页
Chinese Journal of Neurosurgery
关键词
颅脑损伤
免疫耐受
抗脑抗体
Craniocerebral trauma
Immune tolerance
Antibrain antibody
