摘要
目的:研究银杏叶提取物(EGb761)对过氧化氢诱导的大鼠真皮成纤维细胞氧化应激的保护作用。方法:原代培养大鼠真皮成纤维细胞,以不同浓度(20μg/mL、50μg/mL、100μg/mL)的EGb761对其生长进行干预后,H2O2诱导其损伤,MTT法观察细胞活力,硫代巴比妥酸(TBA)法和黄嘌呤氧化酶法分别测定丙二醛(Malondialdehyde,MDA)和超氧化物歧化酶(Superoxide dismutase,SOD)含量;荧光探针DCFH-DA进行活性氧(ROS)检测;流式细胞术(FCM)测定细胞凋亡。结果:不同浓度(20μg/mL、50μg/mL、100μg/mL)的EGb761均能提高H2O2诱导的成纤维细胞细胞存活率和SOD活力,降低MDA含量、活性氧水平和细胞凋亡率,且呈一定的剂量相关性。结论:EGB761在一定范围内能保护过氧化氢诱导的大鼠真皮成纤维细胞氧化应激损伤且呈一定的剂量相关性。
Objective:To investigate the protective effect of EGb761 on hydrogen peroxide-induced oxidative stress injury in fibroblast.Methods:The primary rat dermis fibroblasts were isolated and cultured in vitro,which were treated with Ginkgo biloba extract in different concentration(20mg/L,50mg/L,100mg/L)in exponential phase of cell growth and then exposed to hydrogen peroxide.The cell viability was determined using MTT assay.Superoxide dismutase(SOD)activity and malonyldialdehyde(MDA)content were measured by spect...
出处
《中医药学报》
CAS
2009年第6期29-32,129,共5页
Acta Chinese Medicine and Pharmacology
基金
湖北省教育厅技术创新项目(080-094248
J20061003)
