摘要
目的：建立复方银杏叶刺梨胶囊银杏黄酮醇苷的HPLC测定方法。方法：色谱柱为汉邦C18柱（200 mm×46 mm，5 μm）；流动相为甲醇-04%磷酸水溶液（50∶〖KG-＊2/3〗50）；流速为10 mL·min-1；检测波长为360 nm；柱温为30 ℃。结果：槲皮素、山柰素、异鼠李素分别在128~638 μg·mL-1、129~644 μg·mL-1、26~132 μg·mL-1线性关系良好，平均回收率槲皮素为9948 %，山柰素为10269%，异鼠李素为10015 %。结论：该测定方法简便易行、准确性好、灵敏度高、重复性好，可用于复方银杏叶刺梨胶囊中银杏黄酮醇苷的质量控制。
Objective：To determine the content of flavonol glycosides in Compound Ginkgo and Rosa roxburghii capsuleMethods：The HPLC method was established for the determination of the flavonol glycosides in Compound Ginkgo and Rosa roxburghii capsuleThe HPLC was performed on Hanban C18（46 mm×200 mm，5 μm）column，mobile phase consisted methanol -04% phosphoric acid（50∶〖KG-＊2/3〗50）column temperature 30 ℃，and flow rate was 10 mL·min-1The detection wavelength was 360nmResults：A good linearity was obtained in the range of 128 638 μg·mL-1of quercetin，129 644 μg·mL-1 of kaempferide and 26 132 μg·mL-1 of isorhamnetinThe average recovery rates of quercetin，kaempferide and isorhamnetin were 9948%，10269 % and 10015 %，respectivelyConclusion：The established method is simple，accurate and sensitive，and suitable for quality control of Compound Ginkgo and Rosa roxburghii capsule
出处
《中国现代中药》
CAS
2015年第1期54-57,共4页
Modern Chinese Medicine
基金
江苏高校优势学科建设工程资助项目(2011ZYX6-010)
关键词
复方
银杏叶刺梨胶囊
黄酮醇苷
含量测定
Compound
Ginkgo and Rosa roxburghii capsule
flavonol glycosides
determination
